| 海南龙血树的组织培养与快速繁殖 |
| |
| 引用本文： | 李克烈,李志英,徐立,马千全,罗联忠,黄碧兰,陈伟.海南龙血树的组织培养与快速繁殖[J].热带农业科学,2006,26(5):21-23. |
| |
| 作者姓名： | 李克烈 李志英 徐立 马千全 罗联忠 黄碧兰 陈伟 |
| |
| 作者单位： | 中国热带农业科学院热带作物品种资源研究所,海南,儋州,571737 |
| |
| 基金项目： | 国家科技基础条件平台重点项目“热带作物种质资源收集、整理、保存和保护关键技术和标准研究(2003DIA3N022)”。 |
| |
| 摘 要： | 用种子作外植体,建立了海南龙血树(DracaenacambodianaPierreexGagnepain)的组织培养和再生体系。结果表明:海南龙血树种子诱导愈伤组织的最佳培养基为MS 6-BA3mg/L NAA0.3mg/L,诱导率达80%;附加6-BA2mg/L NAA0.2mg/L的MS培养基有利于芽的分化和增殖,培养60d左右,每个外植体分化形成的再生小植株数达8～20个;在1/2MS NAA0.5mg/L的培养基中诱导生根效果最好。
|
| 关 键 词： | 海南龙血树 组织培养 快速繁殖 |
| 收稿时间： | 2006-04-30 |
| 修稿时间： | 2006年4月30日 |
Tissue Culture and Rapid Propagation of Dracaena cambodiana |
| |
| LI Kelie,LI Zhiying,XU Li,MA Qianquan,LUO Lianzhong,HUANG Bilan,CHEN Wei.Tissue Culture and Rapid Propagation of Dracaena cambodiana[J].Chinese Journal of Tropical Agriculture,2006,26(5):21-23. |
| |
| Authors: | LI Kelie LI Zhiying XU Li MA Qianquan LUO Lianzhong HUANG Bilan CHEN Wei |
| |
| Institution: | Tropical Crops Genetic Resources Institute, CATAS, Danzhou, Hainan 571737 |
| |
| Abstract: | The seeds of Dracaena cambodiana Pierre ex Gagnepain were used as explants to establish a tissue culture and regeneration system. The best medium for callus induction from seeds was found to be the MS 3 mg/L 6-BA 0.3 mg/L NAA. The induction frequency was 80%. The MS supplemented with 2 mg/L 6-BA and 0.2 mg/L NAA was favorable to differentiation and proliferation of buds, and each explant regenerated 8~20 plantlets about 60 days of culture. The optimum root-inducing medium was 1/2 MS supplemented with NAA 0.5 mg/L. |
| |
| Keywords: | Dracaena cambodiana Pierre ex Gagnep tissue culture rapid propagation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
