原位PCR及其在植物研究中的应用

原位PCR是继PCR技术发明以来,分子生物学技术上又一个革命性的突破。由于该技术可检测出组织或细胞中低拷贝,甚至是单拷贝的特异序列,而被越来越多的研究人员青睐,但它在植物上的应用报道较少。该文简要介绍了原位PCR的技术原理;综述了国内外研究人员以植物材料为研究对象进行原位PCR的技术操作;综述了原位PCR在植物细胞水平、组织水平和染色体水平的应用进展;探讨了原位PCR在植物上应用难的原因;从4个方面阐述了原位PCR在植物上的应用前景:(1)单拷贝、低拷贝和多拷贝的基因或特异序列的检测;(2)杂种中亲本染色体的鉴定;(3)物种进化的研究;(4)遗传转化材料的分析。

Advances on Plant in situ PCR Research

The PCR in situ is another revolutionary breakthrough in the technology of Molecule Biology after the PCR was invented. This technology was less reported to apply in plants although it is favoured by more and more researchers due to the fact that it can be used to detect low copies and even single copy of specific sequence in tissue or cell. This paper briefly introduced the principle of the technology of PCR in situ, reviewed the researches on the technical operation of plant in situ PCR in China and abroad, and on the advances on application of PRC in situ in plant cell levels, histological level and chromosome level, and discussed the reasons for difficulty in application of this technology in plants. The future application of the PCR in situ in the plant was elucidated in 4 fields: (1) detection of the single, low and more copies of gene or specific sequence; (2) identification of the parents' chromosomes in hybrids; (3) study on the evolution of plant species, and; (4) analysis of genetically transformed materials.