| 香蕉雄花和组培苗假茎胚性愈伤组织诱导的初步研究 |
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| 引用本文： | 林志聪,赵辉,曾会才.香蕉雄花和组培苗假茎胚性愈伤组织诱导的初步研究[J].热带农业科学,2012,32(1):31-35. |
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| 作者姓名： | 林志聪 赵辉 曾会才 |
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| 作者单位： | 1. 海南大学,海南海口571101／中国热带农业科学院热带生物技术研究所／农业部热带作物生物技术重点开放实验室,海南海口571101
2. 中国热带农业科学院热带生物技术研究所／农业部热带作物生物技术重点开放实验室,海南海口,571101 |
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| 基金项目： | 中国热带农业科学院院本级基本科研业务费项目(No.1630012011004);中国热带农业科学院热带生物技术研究所基本科研业务费项目(No.ITBBZX0822) |
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| 摘 要： | 从外植体来源、生长调节剂种类及浓度等方面研究影响巴西蕉胚性愈伤组织诱导的因素。结果表明,以未成熟雄花为外植体,MS培养基+1 mg/L 2,4-D+1 mg/L IAA+1 mg/L NAA较适宜胚性愈伤组织诱导,26℃暗培养条件下,未成熟雄花的胚性愈伤组织诱导率为3%～4%。在MS培养基+0.7 mg/L 2,4-D+4 mg/L 6-BA中,以不同品种假茎为外植体的脱分化愈伤组织诱导率最高可达75%,但只有巴西(青杆)和海南本地米蕉可以诱导出胚性愈伤组织,诱导率分别为0.1%和0.5%.
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| 关 键 词： | 香蕉 胚性愈伤组织 雄花 假茎 |
Preliminary Study of Embryogenic Callus Induction using Immature Banana Male Flowers and Stems of Tissue Culture Seedlings |
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| LIN Zhicong,ZHAO Hui,ZENG Huicai.Preliminary Study of Embryogenic Callus Induction using Immature Banana Male Flowers and Stems of Tissue Culture Seedlings[J].Chinese Journal of Tropical Agriculture,2012,32(1):31-35. |
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| Authors: | LIN Zhicong ZHAO Hui ZENG Huicai |
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| Institution: | 1 Hainan University,Haikou,Hainan 571101; 2 Institute of Tropical Bioscience and Biotechnology / Ministry of Agriculture Key Laboratory of Tropical Crop Biotechnology,Chinese Academy of Tropical Agricultural Sciences, Haikou,Hainan 571101) |
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| Abstract: | An attempt was made to study factors which affect induction of embryogenic callus derived from the banana cultivar Brazil(Musa AAA Cavendish Subgroup cv.Brazil),including source of explants,plant hormones and concentration of plant hormones.Explants from immature male flowers were cultured on the MS at the presence of 2,4-D 4 mg/L + IAA 1 mg/L + NAA 1 mg/L and incubated in the dark at 26℃.The callus inducing rates were 3%~4% for immature male flowers.In the MS+2,4-D 0.7 mg/L+6-BA 4 mg/L,the different varieties of stems as explants for callus induction rate of up to 75%.However,only Banana Brazil(blue bar) and Hainan powder coke can induce embryogenic callus,induction rate was 0.1% and 0.5%. |
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| Keywords: | banana embryogenic callus male flowers stem |
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