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| 柱花草花粉活力的测定方法 | |
| 引用本文： | 贺庆梅,张华,吴泽霖,罗丽娟.柱花草花粉活力的测定方法[J].热带农业科学,2012,32(7):36-43. |
| 作者姓名： | 贺庆梅 张华 吴泽霖 罗丽娟 |
| 作者单位： | 1. 海南大学园艺园林学院,海南海口,570228 2. 海南大学环境与植物保护学院,海南海口,570228 3. 海南大学农学院,海南海口,570228 |
| 基金项目： | “国家牧草产业技术体系”之“热带牧草育种项目”(No.CARS-35);海南大学“211工程”作物遗传育种学科研究生创新平台建设课题(No.YJSPT-2011-0301) |
| 摘 要： | 利用6种常用花粉活力测定方法对柱花草花粉活力进行测定,并筛选出各方法的最佳配方。结果表明:以离体萌发法为最佳,最适培养基:10%蔗糖、10%PEG-6000(聚乙二醇)、0.01%H3BO3、0.03%CaCl2.2H2O、0.01%KNO3,pH 6.5,柱花草花粉在该培养基上30℃条件下培养10 min,萌发率可达84.03%。0.5%I2+1.5%KI的碘-碘化钾染色效果好,色差明显,较离体萌发法测定值偏高,但差异不显著,可选作简单快速测定法;FDA(荧光素二醋酸脂)染色法、醋酸洋红染色法、无机酸法、TTC(2,3,5-氯代三苯基四氮唑)法均不适合柱花草花粉活力的检测。 |
| 关 键 词： | 柱花草 花粉活力 测定 |
Methods to Test Pollen Viability of Stylosanthes |
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| HE Qingmei,ZHANG Hua,WU Zelin,LUO Lijuan.Methods to Test Pollen Viability of Stylosanthes[J].Chinese Journal of Tropical Agriculture,2012,32(7):36-43. | |
| Authors: | HE Qingmei ZHANG Hua WU Zelin LUO Lijuan |
| Institution: | 1 College of Horticulture and Landscaping,Hainan University,Haikou,Hainan 570228; 2 College of Environment and Plant Protection,Hainan University,Haikou,Hainan 570228; 3 College of Agricultural,Hainan University,Haikou,Hainan 570228) |
| Abstract: | Used 6 commonly methods of pollen viability determination on Stylo pollen viability,and screened out their optimal formula.The results showed that pollen germination in vitro method was the best,and the best medium was 10% sucrose,10% PEG-6000(polyethylene glycol),0.01% H2BO3,0.03% CaCl2,2H2O,0.01% KNO3 and pH 6.5,Stylo pollen germination in this medium at 30℃ for 10 min,and then pollen germination rate were 84.03%.It can be concluded that 0.5% I2 and 1.5% KI components of I-KI staining was the best staining method,because it got no significant difference viability rate to germination in vitro method and can dye fast and clearly.The FDA(fluorescein diacetate) staining,acetocarmine staining,inorganic acid method,TTC(2,3,5-Triphenyltetrazolium chloride) staining were unsuitable to assay pollen vitality of Stylo. |
| Keywords: | Stylosanthes pollen viability determination |
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