PEG模拟干旱胁迫对聚合草叶片生理特性的影响

为研究PEG模拟干旱胁迫环境下聚合草叶片的生理变化,设置不同浓度的PEG溶液(0%、5%、10%、15%、20%、25%)进行处理,对其可溶性糖含量、可溶性蛋白含量、游离脯氨酸含量、过氧化物酶活性、超氧化物歧化酶活性进行测定。结果表明,随着PEG浓度(0%~25%)的增加,可溶性糖含量、可溶性蛋白质含量、POD活性、SOD活性均呈现先上升后下降的趋势。当PEG浓度上升到10%时,POD和SOD活性达到最高值,分别为270.3 U/(g·min)、1167.7 U/g;可溶性糖和可溶性蛋白质含量达到最高值,分别为1.9 μg/g、236.2 μg/g。游离脯氨酸含量随着PEG浓度的升高表现为先下降后上升的趋势,当浓度为10%时,脯氨酸含量到达最低值20.3 μg/g。研究结果表明聚合草具有一定的抗旱性,在轻度干旱环境下通过调节能力可以缓解自身受到的伤害。

PEG Simulating Drought Stress: Effect on Physiological Characteristics of Symphytum officinale Leaves

To study the physiological changes of Symphytum officinale leaves under PEG simulating drought stress, different concentrations of PEG (0%, 5%, 10%, 15% 20%, 25%) were set up. The soluble sugar content, soluble protein content, free proline content, peroxidase activity and superoxide dismutase activity were measured. The results showed that with the increase of PEG concentrations (0%~25%), the soluble sugar content, the soluble protein content, POD activity and SOD activity increased first and then decreased. When the PEG concentration increased to 10%, POD activity and SOD activity reached the highest value, 270.3 U/(g·min) and 1167.7 U/g, respectively; the soluble sugar content and the soluble protein content was up to the maximum, 1.9 μg/g, 236.2 μg/g, respectively. The free proline content decreased first and then increased with the increase of PEG concentrations. When the concentration was 10%, the proline content reached the lowest value of 20.3 μg/g. It indicates that Symphytum officinale has a certain drought resistance, and it could alleviate the damage suffered through own adjustment ability in mild drought environment.