不同品种及产地木耳中多糖含量比较研究

建立木耳多糖新的测定方法,比较不同品种及产地木耳多糖含量的差异,为木耳的质量评价提供依据。采用蒽酮-硫酸法分别测定黑木耳、毛木耳不同产地的多糖含量,并进行方法学考察。葡聚糖在3.27~23.61 μg/mL范围内,其浓度与对应的吸光度呈良好的线性关系(R²=0.9993),平均回收率为101.2%,RSD为3.5%(n=9)。浙江江山、辽宁本溪、福建漳州产黑木耳中多糖含量分别为4.1%、3.0%和3.5%;浙江江山和江苏徐州产毛木耳中多糖含量分别为4.1%和3.6%。蒽酮-硫酸法简便可行、重复性好,适用于木耳中多糖含量的测定。不同产地的黑木耳多糖含量有一定的差异,不同产地的毛木耳多糖含量差异不明显,同产地或地域位置相近的不同产地黑木耳与毛木耳品种间的多糖含量无明显差异。

Auricularia of Different Varieties and Habitats: A Comparative Study on the Polysaccharides Content

The paper aims to establish a method for the determination of polysaccharides in Auricularia and compare the polysaccharide content of Auricularia in different varieties and regions. Anthrone-sulfuric acid method was used to determine the content of polysaccharides in Auricularia auricula and Auricularia polytricha from different habitats, and the methodological investigation was conducted. The results showed that: the concentration of dextran had a good linear relationship with its absorbance in the range of 3.27-23.61 μg/mL (R²=0.9993), with an average recovery rate of 101.2% and RSD of 3.5% (n=9). The polysaccharides content of A. auricula from Jiangshan, Benxi and Zhangzhou was 4.1%, 3.0% and 3.5%, respectively; the polysaccharides content of A. polytricha from Jiangshan and Xuzhou was 4.1% and 3.6%, respectively. The method is suitable for the determination of polysaccharides in Auricularia. There was certain difference in the polysaccharides content of A. auricula from different habitats, but not for the A. polytricha. The overall difference in the polysaccharides content between the two varieties was not obvious.