| 核桃青皮提取物对4种植物枯萎病菌的抑菌作用研究 |
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| 引用本文： | 乔永刚,牛颜冰,乔木,王德富,陈芳.核桃青皮提取物对4种植物枯萎病菌的抑菌作用研究[J].农学学报,2011(7). |
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| 作者姓名： | 乔永刚 牛颜冰 乔木 王德富 陈芳 |
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| 作者单位： | 山西农业大学生命科学学院;山西省和顺县农业局; |
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| 基金项目： | 国家自然科学基金项目“RNA沉默介导的转基因番茄双抗黄瓜花叶病毒和烟草花叶病毒机制研究”(30571216); 山西省自然科学基金项目“细菌表达的病原双链RNA抗相关植物病毒研究”(20051071) |
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| 摘 要： | 为了研究核桃青皮提取物对4种植物枯萎病菌的抑菌作用,笔者采用甲醇、氯仿、石油醚对核桃青皮进行提取,并对抑菌活性最高的提取物(甲醇提取物)进行初步萃取分离,测定了不同溶剂粗提物和甲醇提取物不同溶剂萃取液对茄子枯萎病菌、黄瓜枯萎病菌、辣椒枯萎病菌和西瓜枯萎病菌的抑菌活性。结果表明,核桃青皮甲醇粗提液对4种供试枯萎病菌的抑制效果最好,浓度为4.0mg/mL时,对黄瓜、茄子、辣椒枯萎病菌的抑制率均在60%以上,对西瓜枯萎病菌也为37.9%;核桃青皮甲醇提取物石油醚萃取物对西瓜枯萎病菌的抑制效果最好,抑制率为65%,对菌丝生长的EC50为4.45mg/mL;氯仿萃取物对黄瓜、茄子枯萎病菌的抑制率最高,分别为65%、62.3%,对菌丝生长的EC50分别为4.46、3.51mg/mL;甲醇萃取物对辣椒枯萎病菌的抑制效果较好,抑制率为63.94%,对菌丝生长的EC50为4.23mg/mL。
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| 关 键 词： | 核桃青皮 提取物 抑菌活性 |
The Inhibitory Effect of Extracts from Walnut Peel on 4 Kinds of Plant Fusarium Wilt Pathogens |
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| Qiao Yonggang ,Niu Yanbing ,Qiao Mu ,Wang Defu ,Chen Fang.The Inhibitory Effect of Extracts from Walnut Peel on 4 Kinds of Plant Fusarium Wilt Pathogens[J].Journal of Agriculture,2011(7). |
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| Authors: | Qiao Yonggang Niu Yanbing Qiao Mu Wang Defu Chen Fang |
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| Institution: | Qiao Yonggang 1,Niu Yanbing 1,Qiao Mu 1,Wang Defu 1,Chen Fang 2(1 College of Life Sciences,Shanxi Agricultural University,Taigu 030801,Shanxi,China,2 Heshun Agricultural Bureau of Shanxi Province,Heshun 032700,China) |
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| Abstract: | In order to study the inhibitory effect of extracts from walnut peel on 4 kinds of plant fusarium wilt pathogens,petroleumether,chlorform and methanol were used to extract the walnut peel,the antimicrobial activity of different extracts were tested with Fusarium oxyspoumf.sp.melongenae,Fusarium oxysporiumf.sp.c ucumeris,Fusarium oxysporumf.sp.pepper and Fusarium oxysporiumf.sp watermelon,respectively.The results showed that the methanol extracts of walnut peel had the strongest inhibitory effect on tested f... |
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| Keywords: | Walnut Peel Extracts Antimicrobial Activity |
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