| 一株亚硝酸盐降解菌的分离鉴定 |
| |
| 引用本文： | 周冬仁,沈锦玉,潘晓艺,叶雪平,徐洋,尹文林.一株亚硝酸盐降解菌的分离鉴定[J].农学学报,2011(4). |
| |
| 作者姓名： | 周冬仁 沈锦玉 潘晓艺 叶雪平 徐洋 尹文林 |
| |
| 作者单位： | 浙江省淡水水产研究所; |
| |
| 基金项目： | 湖州市科技局“水质改良有益微生物制剂的研究与开发”(2006C10036) |
| |
| 摘 要： | 为寻找对亚硝酸盐具有高效降解能力、稳定和安全的优良菌株,采用亚硝酸盐氧化细菌(Nitrite-oxidizing bacteria,NOB)富集、分离技术从湖州某水域淤泥中分离得到一株纯培养菌株N3。提取细菌的总DNA,利用细菌16S rDNA特异性引物进行多聚酶链反应(PCR)扩增;扩增产物经琼脂糖凝胶电泳和Sanger末端终止法测序分析,用NCBI-Blast软件将测序结果在Genbank等数据库中进行同源性检索。结果表明,N3的DNA扩增产物片断大小为1441bp,测序结果经检索证实N3与标准菌株Cellulosimicrobium cellulans(AY665978.1)16S rDNA保守性片段有100%的同源性,可以判定所分离得到的N3菌株为纤维单胞菌属的纤维化纤维单胞菌(Cellulosimicrobium cellulans)。
|
| 关 键 词： | 亚硝酸氧化细菌 16SrDNA 聚合酶链反应 同源性分析 |
Isolation and Identification of Nitrite-oxidizing Bacteria |
| |
| Zhou Dongren,Shen Jinyu,Pan Xiaoyi,Ye Xueping,Xu Yang,Yin Wenlin.Isolation and Identification of Nitrite-oxidizing Bacteria[J].Journal of Agriculture,2011(4). |
| |
| Authors: | Zhou Dongren Shen Jinyu Pan Xiaoyi Ye Xueping Xu Yang Yin Wenlin |
| |
| Institution: | Zhou Dongren,Shen Jinyu,Pan Xiaoyi,Ye Xueping,Xu Yang,Yin Wenlin ( Zhejiang Institute of Freshwater Fisheries,Huzhou 313001,Zhejiang,China) |
| |
| Abstract: | In order to isolate good strains with high decomposition ability, stability and safety, a pure culture strain N 3 was isolated from the sludge of a lake in Huzhou, Zhejiang, China by using nitrite-oxidizing bacteria (NOB) preconcentration and separation technique. Total DNAs of N 3 was extracted and specific primers for 16S rDNA of nitrite oxidizer were used to conduct the amplification of polymerase chain reaction (PCR). Amplified product was analyzed by agarose gel e1ectrophoresis and Sanger sequencing. H... |
| |
| Keywords: | Nitrite-oxidizing Bacteria 16S rDNA Polymerase Chain Reaction Homology Analysis |
| 本文献已被 CNKI 等数据库收录！ |
|
