| 福州地区柑橘种质资源的ISSR分析 |
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| 引用本文： | 赵依杰,林丽霞,姚立萍,骆志坚,胡章琼.福州地区柑橘种质资源的ISSR分析[J].农学学报,2020,10(1):72-76. |
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| 作者姓名： | 赵依杰 林丽霞 姚立萍 骆志坚 胡章琼 |
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| 作者单位： | 1. 福州市农业科学研究所,福州 350018;2. 福建省亚热带植物研究所,福建厦门 361006 |
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| 基金项目： | 福建省科技计划项目“福桔种质资源的收集、保存、利用和创新”(2017N0060)，“都市特色园艺作物种质资源保护与创新利用” (2016N3018)。 |
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| 摘 要： | 研究利用ISSR(inter-simple sequence repeat)分子标记技术对21份福州地区柑橘种质资源进行遗传多样性分析,以期为福州地区柑橘的品种鉴定及育种提供理论依据和技术支持。试验从27条ISSR引物中筛选出多态性好、条带清晰的14条引物,对供试材料进行遗传多态性分析。获得162条总条带,其中多态性条带129条,多态位点百分率为79.63%;聚类结果显示21份资源可分为5个组,13份福橘被聚为同一组,进而可划分为4个亚组。ISSR标记能较好地区分福州地区柑橘资源,且福州柑橘地方资源遗传多样性丰富。
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| 关 键 词： | 柑橘 种质资源 福州 ISSR分子标记 遗传多样性 |
| 收稿时间： | 2018/8/23 0:00:00 |
| 修稿时间： | 2019/2/26 0:00:00 |
Genetic Analysis of Citrus Germplasm Resources in Fujian by ISSR Markers |
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| Zhao Yijie,Lin Lixia,Yao Liping,Luo Zhijian,Hu Zhangqiong.Genetic Analysis of Citrus Germplasm Resources in Fujian by ISSR Markers[J].Journal of Agriculture,2020,10(1):72-76. |
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| Authors: | Zhao Yijie Lin Lixia Yao Liping Luo Zhijian Hu Zhangqiong |
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| Institution: | 1. Fuzhou Institute of Agricultural Sciences, Fuzhou 350018, Fujian, China;2. Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian, China |
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| Abstract: | ISSR molecular marker techniques were used to detect the genetic diversity of 21 Citrus germplasms from Fuzhou, to provide a basis for the identification of varieties and the development of new varieties. A total of 162 bands were generated from 14 ISSR primers, of which polymorphic bands were 129, and the polymorphism percentage was 79.63%. The results indicated that, as analyzed by NTSYS-pc2.10e, all the 21 resources could be divided into five groups, 13 Fuzhou Citrus resources were clustered into the same group, and then could be divided into four subgroups. Citrus germplasm from Fuzhou can be distinguished by ISSR markers, and the genetic diversity of Citrus in Fuzhou is rich. |
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| Keywords: | Citrus Fuzhou ISSR genetic diversity |
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