| 燕麦D基因组特异标记开发 |
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| 引用本文： | 翟志文,柴国师,候莎莎,剌士潇,靳艳婷,贾举庆.燕麦D基因组特异标记开发[J].农学学报,2015,5(10):88-92. |
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| 作者姓名： | 翟志文 柴国师 候莎莎 剌士潇 靳艳婷 贾举庆 |
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| 作者单位： | 山西农业大学农学院,电子科技大学生命科学技术学院,山西农业大学农学院,山西农业大学农学院,山西农业大学农学院,山西农业大学农学院 |
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| 基金项目： | 山西省普通高等学校大学生创新创业训练项目“野生燕麦基因组特异标记的开发与应用”(2012075)；山西农业大学大学生科技创新项目“野生燕麦基因组特异标记的开发与应用”(10-004)；山西农业大学引进人才科研启动项目“燕麦基因组中反转录转座子的分离与应用”(XB2010009)。 |
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| 摘 要： | 为了提高燕麦分子育种的效率,旨在开发燕麦D基因组特异分子标记。先利用300条10碱基随机引物对9种燕麦种材料进行随机多态性扩增(random amplified polymorphic DNA,RAPD),扩增出燕麦D基因组特异性条带,命名为OPAB011287,经Blast比对后发现该条带为一反转座子重复序列,根据此条带序列设计特异性引物,建立更简便稳定的SCAR标记。结果发现该SCAR标记可以准确特异的检测到9种试验材料中含有D基因组的燕麦品种,结论表明该标记可用于燕麦分子标记辅助育种,有效服务于燕麦优良品种培育。
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| 关 键 词： | 燕麦 D基因组 重复序列 分子标记 |
| 收稿时间： | 2015/5/12 0:00:00 |
| 修稿时间： | 2015/5/26 0:00:00 |
Development of D Ggenome-specific Marker in Oat |
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| Abstract: | The research aims to improve the efficiency of molecular breeding and develop D- Genome-specific markers in oat. Firstly, random amplified polymorphic DNA (RAPD) analysis was performed on 9 oat varieties with 300 random 10-based primers. A repetitive sequence of 1287 bp, named OPAB011287 was identified in the D genome of oat. BLAST search revealed that the sequence of OPAB011287 was highly homologous to a part of a retrotransposon. Based on the sequence of OPAB011287, a pair of sequence- characterized amplified region primers (SCAR) was designed which was more convenient and stable. And the results showed that SCAR marker was specific to target oat with D genome, which provided a powerful tool for oat marker assisted breeding and the better service for the breeding of oat. |
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| Keywords: | Oat D Genome Repetitive Sequence Molecular Marker |
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