菠萝混合授粉杂交品系表型研究及其父本来源分析

旨在研究菠萝混合授粉杂交品系‘H’的父本来源。比较‘H’与其母本以及混合花粉供体的表型性状；并基于表型性状遗传距离分析及SLAF测序，研究‘H’与混合花粉供体间的遗传相似性及SNP基因型来源。杂交品系‘H’的物候期、植株大小，果实理化品质、色泽等表型性状与花粉供体之一的‘神湾’相当，而部分理化品质指标优于‘神湾’；果实香气物质组分及含量与其母本‘无刺卡因’较接近。果实小于其母本‘无刺卡因’及‘巴厘’，而大于‘神湾’。杂交品系‘H’与‘神湾’间的遗传相似性大于‘H’与‘巴厘’间的遗传相似性。通过SLAF简化基因组测序分析表明，‘H’的父本来源于‘神湾’的可能性大于‘巴厘’。

Phenotype Analysis of Pineapple Hybrid Line Obtained by Mixed-pollen Cross and Its Paternal Origin Analysis

This work was to investigate the paternal origin of pineapple hybrid line H obtained by mixed-pollen cross. In this work, the phenotypes were investigated by comparing the growth characteristics and quality properties of H with its female parent as well as the two pollen supporters. Additionally, the genetic similarity and SNP gene source of H with its female and the two pollen supporters were calculated based on euclidean distance analysis and SLAF-seq. The obtained results suggested that the phenotypic parameters such as the phenology, plant size, as well as the quality and color aspects of fruit were quite alike to those of Yellow Mauritius, which was one of the pollen supporters, and some physical-chemical characteristics of H excelled. The aroma components of H were similar to those of female parent Smooth Cayenne. The size of fruit of H was lower than that of Smooth Cayenne and Comte de.Paris, while higher than Yellow Mauritius. The analysis of euclidean distance of phenotype parameters suggested that the genetic similarity between H and Yellow Mauritius was higher than that between H and Comte de.Paris. SLAF-seq showed that the possibility of H''s paternal origin from Yellow Mauritius was greater than that of Comte de.Paris. H''s paternal origin was probably from Yellow Mauritius.