菠萝多糖抗氧化功能的研究

通过从菠萝皮渣中提取菠萝粗多糖,探讨菠萝粗多糖的体外抗氧化功能。采用热水浸提法提取菠萝多糖,用羟自由基体系、超氧阴离子体系、多糖的还原能力体系研究菠萝多糖清除自由基的能力。结果表明,用水杨酸比色法测定菠萝多糖去除羟自由基能力,当浓度为12 mg/mL时,对羟自由基的清除能力达90%以上。用邻苯三酚法测定菠萝多糖清除超氧阴离子的能力,当浓度为6 mg/mL时,超氧阴离子的清除能力达80%以上。菠萝多糖还原能力与浓度呈良好的量效关系。

Study on the Functional Activity of Pineapple Polysaccharide in vitro

Pineapple polysaccharide was extracted from pineapple peel to discuss the antioxidant function of polysaccharide pineapple in vitro. Hot water extraction was used to extract polysaccharide pineapple. The ability of pineapple polysaccharide to scavenge free radicals was studied by using hydroxyl radicals system, superoxide anion system and polysaccharide reductive ability system. Results showed that, using salicylic acid colorimetry to determine the ability of pineapple polysaccharide to scavenge hydroxyl radicals, when the concentration was 12 mg/mL, the scavenge rate reached 90%. While using pyrogallol autoxidation method to determine the ability of pineapple polysaccharide to scavenge the superoxide anion, when the concentration was 6 mg/mL, the clearance of super oxide anion reaches 80%. The reductive ability of pineapple polysaccharide was enhanced with its concentration was increased, and there was a good concentration-response relationship between the concentration and reductive ability.