| 苹果三倍体成熟胚的离体培养研究 |
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| 引用本文： | 何平,李林光,张志宏,焦淑华,孙乃波.苹果三倍体成熟胚的离体培养研究[J].安徽农业科学,2006,34(16):3926-3928. |
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| 作者姓名： | 何平 李林光 张志宏 焦淑华 孙乃波 |
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| 作者单位： | 沈阳农业大学园艺学院,辽宁,沈阳,110161;沈阳农业大学园艺学院,辽宁,沈阳,110161;山东省果树研究所,山东,泰安,271000;内蒙古赤峰农牧学校,内蒙古,赤峰,024005 |
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| 摘 要： | 以苹果三倍体乔纳金成熟胚为材料,在含不同激素或添加物的培养基上进行萌发出苗培养,结果表明:F14+活性炭1.0g/L+水解乳蛋白400mg/L+抗坏血酸100mg/L为最适宜培养基;光照培养比黑暗培养好;培养基中添加0.5mg/L的6-BA和0.2mg/L的NAA能有效促进萌动胚的胚根伸长和发育成苗。
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| 关 键 词： | 苹果三倍体 成熟胚 离体培养 |
| 文章编号： | 0517-6611（2006）16-3926-03 |
| 收稿时间： | 05 16 2006 12:00AM |
| 修稿时间： | 2006-05-16 |
Mature Embryo Culture in Vitro of Triploid Apple |
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| HE Pingetal.Mature Embryo Culture in Vitro of Triploid Apple[J].Journal of Anhui Agricultural Sciences,2006,34(16):3926-3928. |
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| Authors: | HE Pingetal |
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| Institution: | CollegeofHorticulture,ShenyangAgricuhural University,Shenyang,Liaoning 110161 |
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| Abstract: | The mature embryos of triploid apple(Malus domestica Borkh.)of Jonagold were cultured on the medium supplemented with different additional components or plant hormones. The result showed:the best medium was F14 containing activated carbon 1.0 g/L,lactalbumin hydrolysate 400 mg/L and ascorbic acid 100 mg/L.The cultured mature embryos grew better in the light than in dark. The radicle grew and formed plantlet from the budded mature embryos that were cultured on the medium contained 0.5 mg/L 6-BA and 0.2 mg/L NAA. |
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| Keywords: | Triploid apple Mature embryo Culture in vitro |
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