螺旋藻藻蓝蛋白在水和磷酸缓冲液中稳定性的对比研究

目的]为了寻找一种代替磷酸缓冲液的提取溶液，满足尽量保持其活性的要求。方法]分别用蒸馏水和磷酸缓冲液，采用研磨法提取藻蓝蛋白并保存。结果]两者提取效果差别不大。但是，水保存的藻蓝蛋白溶液比磷酸缓冲液保存的藻蓝蛋白溶液易分解。结论]综合试验条件及藻蓝蛋白稳定性影响因素考虑，可确定pH变化为藻蓝蛋白分解的主要原因；蒸馏水可代替磷酸缓冲液用于提取藻蓝蛋白，活性维持时间不超过15d。

Comparison of Stability of Spirulina Phycocyanin in Distilled Water and Phosphate Buffer

Objective ] The research aimed to explore a new solution rather than phosphate buffer to extract phycocyanin with remaining activ- ity as far as possible. Method] The phycocyanin in Spirulina platensis was extracted with distilled water and phosphate buffer, and then re- stored respectively. ~ Result] There was no sharp difference between the two extractive results. Yet, pH of the extraction of phycocyanin in distilled water changed more and decomposed faster than that in phosphate buffer. Conclusion ] Considering all of the stability-influencing factors, pH played the most significant role in this experiment. Distilled water could be used to extract phycocyanin and the extractive accepta- ble time wasn＇t more than 15 days.