LPS致肝细胞损伤模型的建立研究

目的]探索LPS体外致人ChangLiver细胞损伤模型的建立方法和意义。方法]分别用20．40、60、80、1130μg／ml浓度的LPS作用ChangLiVer细胞24h，采用MTF法检测LPS对ChangLivet细胞存活率的影响，分别测定ChangLiver细胞上清中谷草转氨酶（AST）、谷丙转氨酶（ALT）、碱性磷酸酶（ALP）、γ-谷氨酰转肽酶（γ-GT）、乳酸脱氢酶（IJDH）的活性，并通过Hoechst33258荧光染色观察用药24h后ChangLiver细胞形态的变化。结果]80和100u晷／ml浓度的LPS作用后ChangLiver细胞存活率显著性降低，胞外AST、ALT、ALP、γ-GT、LDH酶活性升高，细胞数目减少，细胞形态发生改变，呈现细胞核固缩等细胞凋亡现象。（结论l用80μg/ml的LPS与ChangLiver细胞共培养24h，可以建立理想的体外肝细胞损伤模型。

To Establish and Research Liver Cell Injury Model Induced by LPS

Objective ] To establish in vitro LPS-induced Chang Liver cells damage model Method] After received LPS 24h at different con- centrations （20, 40, 60, 80, 100 μg/ml）, Chang Liver cells viability effects were detected by MTT methods, and the AST, ALT, ALP,γ-GT, LDH activity in ceU supernatant were measured. The Chang Liver cells morphology changes were showed by Hoechst 33258 staining. Result] Chang Liver cell viability was significantly reduced with LPS in 80 and 100 μg/ml. AST, ALT, ALP, γ-GT, LDH activity in cell supernatant were increased; the number of ceils were reduced; ceU morphology was changed showing cell apoptosis with nuclear condensation. Conclusion ] Chang Liver ceils co-cultured with 80μg/ml LPS might be an ideal in vitro model of hepatocellular injury.