一株产乙醇嗜热厌氧油藏微生物Thermoanaerobacter sp DF3的性质研究

目的]了解油藏微生物Thermoanoerobacter sp DF3的生理生化特性，优化木糖产乙醇培养方案。方法]利用厌氧分离技术从大港油田油层采出液中分离到一株产乙醇厌氧杆菌DF3。采用生理生化鉴定与16SrDNA序列的系统发育学分析其系统发育地位。用气相色谱分析其代谢产物。结果]菌株DF3是一株严格厌氧的嗜热细菌，呈直杆状，G^-，菌体大小为O．42μm×（1．60～5．20）μm，单生、成对或成串生长，产顶端芽孢；生长温度为45～78℃（最适65℃），能利用葡萄糖、木糖、果糖、核糖、甘露糖、阿拉伯糖、蔗糖、半乳糖、乳糖、纤维二糖、松三糖、棉子糖、淀粉等作为底物；其16SrRNA与zpseudoethanolicus相似性为99．7％。发酵葡萄糖与木糖的主要产物为乙醇，培养方案优化后其代谢木糖产乙醇终浓度为2．og／L。结论]通过试验证明菌株DF3是目前已知菌株中产乙醇活性较强的茵株之一，在65℃时代谢木糖能产生2．Og／L的乙醇。目前代谢木糖高产乙醇的茵株均由国外分离获得，菌株DF3的分离获得为我国研究木质纤维素产乙醇提供了优良的出发菌株。

Research on the Nature of a Thermophilic Anaerobic Ethanol Producer Thermoanaerobacter DF3 in Oil Pool

Objective]The research aimed to learn about the physiological and biochemical characteristics of the microbe Thermoanaerobacter sp DF3 in oil pool and optimize the culture plan of producing ethanol from xylose.Method]A strain of anaerobic ethanol-producing bacillus DF3 was isolated from produced liquid from oil layer of Dagang oil field using anaerobic isolation technics.Physiological and biochemical identification and phylogenetic analysis of 16S rDNA sequence were used to analyze its phylogenetic positi...