高效提取肠道菌群总DNA方法的建立

目的]建立一种高效快速的提取肠道茵群总DNA的方法，即改进的氯仿抽提法，为进一步对肠道茵群的定性和定量提供基础。方法]通过对多种肠道菌群总DNA提取方法的考察总结，对氯仿抽提法进行改进，采用细菌添加试验的荧光实时PCR检测抽提结果，同时与QIAamp DNA Stool Mini kit法进行比较，以验证所建立的改进的氯仿抽提法的高效性。结果]改进的氯仿抽提法提取的肠道茵群总DNA量约是QIAamp DNA Stool Mini kit的100倍；同时细菌添加试验的实时荧光定量PCR结果显示，改进的氯仿抽提法提取添加细菌的DNA效率较高。结论]改进的氯仿抽提法经济、高效、快速，适合大批量的粪便样品的DNA提取。

Development of an Efficient Method for Extracting Total DNA of Intestinal Microflora

Objective]The aim was to develop a fast and effective DNA extraction method of intestinal microflora,a modified method of chloroform extraction,and to provide the basis for distinguishing these bacteria.Method]Through the improvement of conventional DNA extraction method,a rapid and efficient DNA extraction method was developed.Compared with the real-time PCR result of control sample and the result of QIAamp DNA Stool Mini kit,the developed method was verified.Result]The DNA yield of the developed method...