水芹水浸提液对小球藻生长及抗氧化酶活性的影响

目的]研究水芹水浸提液对小球藻生长及其抗氧化酶活性的影响。方法]采用小球藻在不同浓度的水芹水浸提液中纯培养的方法,测定小球藻生长速率、超氧化物歧化酶（SOD）和过氧化物酶（POD）活性、·O2-产生速率及丙二醛（MDA）含量的变化。结果]10g/L水芹水浸提液处理下,SOD和POD活性逐步上升,至试验结束时都维持在较高的水平,·O2-产生速率和MDA含量与对照间无显著差异;20～50g/L水芹水浸提液处理,藻细胞的SOD、POD活性、·O2-产生速率及MDA含量均呈先升高后降低的趋势,并且有浓度效应。结论]水芹水浸提液对小球藻具有化感效应。10g/L水芹水浸提液对小球藻的生长具有明显的促进效应,20～50g/L水芹水浸提液对小球藻有明显的克生作用。

Effects of Watercress Extracts on Growth and Activities of Antioxidant Enzymes of Chlorella vulgaris

Objective]The effects of watercress extracts on the growth and activities of antioxidant enzymes of Chlorella vulgaris were studied.Method]In pure culturing Chlorella vulgaris with different concentrations of watercress extract the growth,SOD and POD activity,·O2-generating rate and MDA content of Chlorella vulgaris were determined.Result]Under the treatment of 10 g/L watercress extract the activity of SOD and POD increased gradually and maintained upper level in the whole process,·O2-generating rate and the content of MDA didn't have significant differences compared with control.Under the treatment of 20-50 g/L watercress extracts,the activity of SOD and POD,·O2-generating rate and the content of MDA initially increased,then decreased,and intensified with time extension and concentration increase.Conclusion]Watercress extracts had allelopathy on Chlorella vulgaris.On the whole,10 g/L watercress extracts promoted the growth of Chlorella vulgaris significantly,and 20-50 g/L watercress extracts had obvious inhibition.