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德氏乳杆菌对鸡肠道菌群的影响
引用本文:许提森.德氏乳杆菌对鸡肠道菌群的影响[J].安徽农业科学,2008,36(8):3228-3229.
作者姓名:许提森
作者单位:德州学院生物系,山东,德州,253023
基金项目:德州市科学技术攻关项目(042102).
摘    要:目的]探索德氏乳杆菌对鸡肠道菌群的影响。方法]从肉鸡肠黏膜上分离培养制备试验菌株。在45只肉鸡雏苗中投喂添加由单一德氏乳杆菌制备的微生态制剂和土霉素,在55d的投喂期内,采用平板计数法检测好氧性异养菌、鸡伤寒沙门氏菌、金黄色葡萄球菌和德氏乳杆菌的数量。结果]从肉鸡肠黏膜上分离培养得到6株菌,均为G+菌,生理生化鉴定表明,S2为嗜酸乳杆菌,S4为德氏乳杆菌乳亚种,S6为德氏乳杆菌德氏亚种,S1、S3和S5为粪肠球菌。在投喂冻干菌粉30d后德氏乳杆菌数量达到稳定并在肠道内定植。同时,由于德氏乳杆菌的抑制作用,肠道中的鸡伤寒沙门氏菌数量下降最明显。德氏乳杆菌对鸡肠道的好氧性异养菌没有任何影响。结论]德氏乳杆菌对鸡肠道菌群的影响与抗生素具有相近的效果,表明德氏乳杆菌作为饲料添加剂可以取代抗生素应用在肉鸡的养殖中。

关 键 词:德氏乳杆菌  肠道菌群  抗生素
文章编号:0517-6611(2008)08-03228-02
修稿时间:2007年11月28

Effect of Lactobaciilus delbrueckii subsp.delbrueckii on the Micro FIora in Broiler Gut
XU Zhi-Sen.Effect of Lactobaciilus delbrueckii subsp.delbrueckii on the Micro FIora in Broiler Gut[J].Journal of Anhui Agricultural Sciences,2008,36(8):3228-3229.
Authors:XU Zhi-Sen
Abstract:Objective] The aim was to explore the influences of Lactobacillus delbrueckii subsp.delbrueckii on the micro flora in broiler gut. Method] Experimental lactic acid bacteria (LAB) were isolated from intestinal mucous of broiler gut. Forty-five broilers were reared on feed supplement with Lactobacillus delbrueckii subsp.delbrueckii alone and oxytetracycline. After 55 days of feeding,the total numbers of aerobes,Salmonella typhimurium,Staphylococcus aureus and Lactobacillus delbrueckii subsp.delbrueckii were measured by plate colony count. Result] Six strains were isolated from intestinal mucous of broiler gut. According to their physioloical and biochemical characteristics,3 strains were identified as belonging to the genus of Lactobacills:S2 as Lactobacillus asidophilus,S4 as Lactobacillus delbrueckii subsp.lactic,S6 as Lactobacillus delbrueckii subsp.delbrueckii . Other 3 strains were identified as Enterococcus faecalis. After 30 days administrated with lyophilized bacteria,Lactobacillus asidophilus colonized in the broiler gut with stable number. The number of Salmonella typhimurium reduced because of the inhibitory effect of Lactobacillus delbrueckii subsp.delbrueckii. But it had no effects on aerobes in broiler gut. Conclusion] The influences of Lactobacillus asidophilus on the micro flora in broiler gut were similar to those of oxytetracycline. It was suggested that LAB additive could be used in broiler farming as substituting for antibiotics.
Keywords:Lactobacillus delbrueckii  Intestinal micro flora  Antibiotic
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