香茅草叶绿素含量分析

目的]探讨不同浸提液对香茅草叶绿素含量测定的影响。方法]采用分光光度法测定香茅草叶绿素的含量,并采用不同浸提液对比香茅草叶绿素提取效果。结果]丙酮∶甲醇=2∶1混合浸提液对澳洲香茅草叶绿素的提取效果较佳,对爪哇香茅草、柠檬香茅草和泰国香茅草叶绿素的提取丙酮∶乙醇=1∶1混合浸提液效果最佳;4个香茅草品种中,柠檬香茅草的叶绿素含量最高,其次是泰国香茅草,爪哇香茅草和澳洲香茅草的叶绿素含量差异不显著;澳洲香茅草的叶绿素a、叶绿素b和总叶绿素含量均最低。叶绿素a/叶绿素b比值分别为澳洲香茅草3.89、爪哇香茅草4.35、柠檬香茅草4.55和泰国香茅草4.09。结论]泰国香茅草在成林期比澳洲香茅草、爪哇香茅草和柠檬香茅草具有较高的光合性能和生长速率。

Analysis of Chlorophyll Content in Cymbopogon citratus (DC.) Stapf

Objective] The research aimed to study the effect of different extracts on the chlorophyll content of Cymbopogon citratus.Method] The chlorophyll content of Cymbopogon citratus was determined by UV spectrophotometry.And the effect of different extracts on chlorophyll extraction was compared.Result] Acetone∶ methanol =2∶1 mixed extract had a better effect on the extraction of chlorophyll from the Australian Cymbopogon citratus.While the mixed extraction of the acetone and ethanol(1∶1) had the best effect on the extraction of chlorophyll of Javanica Cymbopogon citratus,lemon Cymbopogon citratus and Thailand Cymbopogon citratus;Among these 4 different varieties of Cymbopogon citratus,the content of the chlorophyll was the highest in lemon Cymbopogon citratus,then Thailand Cymbopogon citrates.The difference of chlorophyll content in Javanica Cymbopogon citratus and Australian Cymbopogon citratus was not significant.The chlorophyll-a,chlorophyll-b and total chlorophyll content of Australian Cymbopogon citrates were the lowest among those four types of Cymbopogon citratus.The ratio of Chlorophyll a/chlorophyll b was 3.89 for Australia Cymbopogon citratus,4.35 for Javanica type Cymbopogon citratus,4.55 for lemon Cymbopogon citratus and 4.09 for Thailand Cymbopogon citratus.Conclusion] The Thailand Cymbopogon citratus has higher photosynthetic performance and growth rate among those four types of Cymbopogon citrates.