温度对龙葵幼苗叶绿素含量和荧光参数的影响

目的]研究温度处理对黑果龙葵叶片叶绿素含量和荧光参数的影响。方法]以北方常见黑果龙葵幼苗为试材,设置昼/夜温度(35/30、30/25、25/20、20/15、15/10),通过对叶绿素含量和叶绿素荧光参数的测定,分析不同温度对龙葵幼苗叶绿素含量、叶绿素荧光猝灭特性和光能分配等的影响,研究龙葵幼苗适应性反应以及生理机制。结果]随着温度降低,龙葵幼苗叶绿素a、叶绿素b、叶绿素(a+b)含量、Fv/Fm、ΦPSⅡ、q P均逐渐减小,qN增大,并且随时间的延长增大幅度提高;低温导致龙葵幼苗叶片PSⅡ的部分失活或受到一定伤害,原初光能转换效率下降,电子传递活性减弱,有效抑制了光合碳代谢电子供应效率,从而影响植物的光合作用,并随温度的降低抑制作用加剧。结论]该研究为新型野生蔬菜龙葵的人工栽培提供相关理论依据。

Effects of Different Temperature on the Content of Chlorophyll in the Leaves of Solanum nigrum L.Seedlings and the Fluorescent Index

Objective] The research aimed to study the effects of temperature on the chlorophyll content and fluorescence parameters of Solanum nigrum leaves.Method] Taking the seedlings of Solanum nigrum in the north as test material,the daytime/night temperature (35/30,30/25,25/20,20/15,15/10) were set.Through the determination of chlorophyll content and chlorophyll fluorescence parameters,the effects of different temperature on the chlorophyll content,chlorophyll fluorescence quenching characteristics and light energy allocation of Solanum nigrum were studied,and the adaptive response and physiological mechanism of Solanum nigrum seedlings were studied.Result] With the decrease of temperature,the contents of chlorophyll a,chlorophyll b,chlorophyll (a + b),Fv / Fm,ΦPS Ⅱ and qP in the seedlings of Solanum nigrum decreased gradually and qN increased,and increased with the extension of time.In low temperature,PS Ⅱ in the leaves of Solanum nigrum seedlings will be inactive or damaged partly,the conversion efficiency of the original light energy decreased and the electron transfer activity weakened,which effectively inhibited the photosynthetic carbon metabolism electron supply efficiency and thus affected the photosynthesis of plants and decreased with temperature inhibition intensified.Conclusion] The research provides theoretical basis for the artificial cultivation of the new wild Solanum nigrum.