不同附生树种对金钗石斛石斛碱含量的影响

目的]测定不同附生树种金钗石斛中石斛碱的含量。方法]以贵州赤水仿野生3年苗为试验材料,不同树种继续附生生长1年,采用气相色谱内标法,测定不同附生树种金钗石斛中石斛碱的含量。色谱柱:DB-1(30 m×0.25 mm×0.25μm);程序升温:初始温度80℃,10℃/min升温至250℃,保持5 min;进样口温度250℃;FID检测器温度250℃。结果]不同附生树种金钗石斛中石斛碱的含量有明显差异,其中,杉木附生金钗石斛的石斛碱含量最高,为0.932%,枇杷、杨梅、龙眼附生金钗石斛石斛碱含量分别为0.825%、0.790%和0.601%。结论]不同附生树种的金钗石斛石斛碱含量均符合2015版《中国药典》限量要求,该研究为确定适宜的附生树种提供参考依据。

Effects of Different Epiphyllous Species on Dendrobine of Dendrobiun nobile

Objective] To measure the contents of dendrobine of Dendrobiun nobile from different epiphyllous trees.Method] D.nobile is a kind of plant found in Chishui of Guizhou Province,which attached to different epiphyllous trees to grow for one more year.Gas chromatography internal standarded method was adopted to determine the contents of Dendrobine.An DB-1 (30 m ×0.25 mm ×0.25 μm) column was used,with the programming temperature 80 ℃,and then up to 250 ℃ keeping 5 min with the increasing rate of 10 ℃.The injector temperature was 250 ℃ and the FID detector temperature was 250 ℃.Result] The Dendrobine contents of D.nobile on Cunninghamia lanceolata was 0.932％,0.825％ on loquat,0.790％ on Myrica rubra,0.601％ on Dimocarpus longan.Conclusion] The contents of dendrobine of D.nobile on different epiphyllous trees can meet the requirement of Chinese Pharmacopoeia(2015),the research offered evidence of ideal habitat for the growth of D.nobile.