植物羟基肉桂酰辅酶A还原酶的生物信息学分析

分析GenBank公布的68条蕨类、裸子、单子叶和双子叶植物的CCR蛋白,发现单子叶植物CCR基因的GC含量最高,CCR一级结构的理化性质基本一致,但主要氨基酸种类和含量不同;CCR是一类无导肽、信号肽及跨膜结构域的亲水性蛋白质,N-端存在3β-羟基类固醇脱氢酶/差向异构酶/NAD结合蛋白的结构域,存在9个功能保守区;进化树表明,该基因可用于植物高等级单元的分类;同源建模表明其三级结构稳定,建模结果可靠;CCR蛋白亚细胞定位于细胞质、叶绿体和内质网,除黑麦草和番茄外,同一物种CCR不同成员的亚细胞定位基本相同.

Bioinformatics Analysis of CCR Enzyme in Plant

In order to fully understand the characteristics of the CCR enzyme, 68 CCR genes were obtained from NCBI GenBank database. Bioinformaties methods were used to analyze the physics and chemical characteristics, amino acid composition, conserved domains. The leader peptide, signal peptide, transmembrane domain and hydrophilicity of peptide of CCR protein were analyzed likewise. The phylogenetie tree were constructed and analyzed as well as 3-dimensional structure. The results showed that GC content in monocotyledon was higher than dicoty- ledon or gymnosperm. There were nine conserved domains in the all CCR proteins and they had a highly similarity. The physics and chemical characteristics of CCR proteins were similar, but the kinds and content of primary amino acid were different. And neither the obvious leader or signal peptide nor the transmembrane domain were detected. The data showed that CCR protein was hydrophilieity protein and one 3beta_ HSD/Epimerase/NAD binding_4 domain were existed in the N-terminus. The phyloganetic tree showed that the CCR gene might be used to treat taxa at higher rank in plant. Based on sequence conservation and protein-protein interaction, subeellular localization prediction of protein showed that CCR might mainly be locate in the cytoplasm, followed by the cytoplasm and the endoplasmic retieulum.