溃疡病菌诱导下毛白杨防御相关基因的应答反应

目的]研究溃疡病菌诱导下毛白杨防御相关基因的应答反应.方法]以毛白杨为试验材料,用溃疡病菌对7月龄幼苗进行接种,提取诱导前后的毛白杨树皮mRNA,通过反转录生成cDNA,构建72 h差异表达的SSH-cDNA文库.随机挑选199个阳性克隆进行测序,将测序结果与NCBI基因库中序列进行比较,并对这些EST在dbEST数据库进行同源检索分析,利用RT-qPCR对文库中7个基因进行进一步分析.结果] 172个EST找到了同源序列,其中有32个序列与防御相关.在毛白杨受到溃疡病菌诱导时,这些防御基因的表达量明显上升.结论]该研究为开展毛白杨抗溃疡病重要功能基因的克隆和鉴定奠定了基础.

Response of Defense-related Genes of Populus tomentosa Induced by Botryosphaeria dothidea

Objective ] To study the response of defense-related genes of Populus tomentosa induced by Botryosphaeria dothidea. Methods ] Total RNA was extracted from the bark of Populus tomentosa before and after inoculation by Botryosphaeria dothidea. Barks mRNA were puri- fied and transcribed into cDNA. Constructed a poplar-pathogen interaction cDNA library from fungus inoculated poplar bark 72 h post-inocula- tion （hpi）, randomly selected 199 positive cloning. Selected unisequences defense-related, and further analysis by quantitative real-time PCR （qRT-PCR）. Results] 172 EST sequences showed homology to sequences with assigned putative identity in public databases, including 32 sequences associated with defense-related genes. Conclusion] The expression of these genes was significantly increased in Populus tomentosa induced by Botryosphaeria dothidea. It is expected that this study will lay certain foundation for cloning of major genes of Populus tomentosa having function of resisting Botryosphaeria dothidea.