| 正交试验法优化罗布麻叶中总黄酮的闪式提取工艺 |
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| 引用本文： | 白明,李连珍,王亚宁,王宪龄.正交试验法优化罗布麻叶中总黄酮的闪式提取工艺[J].安徽农业科学,2013(18):7761-7762,7767. |
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| 作者姓名： | 白明 李连珍 王亚宁 王宪龄 |
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| 作者单位： | 河南中医学院,河南郑州,450046;河南农业大学,河南郑州,450002 |
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| 基金项目： | 河南中医学院科技创新支持计划 |
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| 摘 要： | 目的]优化闪式提取器提取罗布麻叶中总黄酮成分的工艺条件。方法]在单因素试验的基础上,运用L9(34)正交试验优选罗布麻叶总黄酮的闪式提取工艺。结果]闪式提取法提取罗布麻叶中总黄酮的最佳工艺为:乙醇浓度55%,料液比1∶50(W/V,g/ml,下同),提取时间50 s,提取次数2次;在此条件下,最高提取率为4.24%。验证性试验用最佳工艺条件提取5次,RSD为1.07%。结论]该工艺稳定可行,闪式提取高效、快速,不破坏成分,有推广价值。
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| 关 键 词： | 罗布麻叶(FOLIUM APOCYNI VENETI) 正交试验 闪式提取 总黄酮 |
The Optimization of Flash-type Extractional Process of Total Flavonoids in Apocynum Venetum Leaves by Orthogonal Experiment |
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| Institution: | BAI Ming( 1.Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046;) |
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| Abstract: | Objective] To explore the best process of flash-type extractor for extracting total flavonoids in Apocynum venetum leaves.Method] Using L9 （34） orthogonal experimental method to optimize the flash-type extractional process of total flavonoids in Apocynum venetum leaves by single factor and orthogonal experiment.Result] The optimal extraction technology is：ethanol concentration 55％,ratio of solid to liquid 1：50,extraction time 50 s,2 times,the highest extraction rate is 4.24％.The optimum technique conditions for verification test is extracting for 5 times,RSD is 1.07％.Conclusion] The process is stable and feasible,suitable for promotion,and the flash-type extraction is efficient,fast and no damage for the components. |
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| Keywords: | Apocynum venetum leaves Orthogonal experiment Flash-type extraction Total flavonoids |
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