| 脂肪酶产生菌黑曲霉ZW-1复合诱变育种研究 |
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| 引用本文： | 朱琪,王维,兰时乐.脂肪酶产生菌黑曲霉ZW-1复合诱变育种研究[J].安徽农业科学,2013(18):7796-7799. |
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| 作者姓名： | 朱琪 王维 兰时乐 |
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| 作者单位： | 湖南农业大学生物科学技术学院,湖南长沙,410128;湖南农业大学生物科学技术学院,湖南长沙,410128;湖南农业大学生物科学技术学院,湖南长沙,410128 |
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| 摘 要： | 目的]对脂肪酶产生菌黑曲霉ZW-1进行复合诱变育种研究。方法]以脂肪酶产生菌黑曲霉ZW-1为出发菌株,通过紫外线、微波及硫酸二乙酯进行复合诱变得到变异株,对筛选出的变异株产生的脂肪酶活力进行测定。结果]试验选育到1株脂肪酶产量较高的变异株8632;在适宜条件下,该菌株产生的脂肪酶活力达104.62 IU/ml,比出发菌株提高了125.86%;遗传稳定性试验表明该突变株具有良好的传代稳定性。结论]该研究为满足脂肪酶在工业生产中的需求提供了科技支持。
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| 关 键 词： | 脂肪酶 诱变育种 黑曲霉 DES 微波诱变 |
Study on Compound Mutation Breeding of Lipase-producing Strain Aspergillus niger ZW-1 |
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| Institution: | ZHU Qi( 1.College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128;) |
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| Abstract: | Objective] The aim was to study the compound mutation breeding of lipase-producing strain Aspergillus niger ZW-1.Method] Using the lipase-producing strain A.niger ZW-1 as starting strain,mutants were obtained and selected by the compound mutation of ultraviolet,microwave and diethyl sulfate （DES）,and the lipase activity of mutants were determined.Result] A mutant strain 8632 was bred with high yield of lipase; the activity of lipase produced by that strain reached 104.62 IU/ml under suitable conditions,and it was about 125.86％ higher than that of the starting strain ; the genetic stability experiment showed that the mutant 8632 had good genetic stability after 5 times of subculture.Conclusion] The study provided scientific support to meet the needs of lipase in industrial production. |
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| Keywords: | Lipase Mutation breeding Aspergillus niger DES Microwave mutation |
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