金钗石斛多糖对脂多糖诱导的小鼠腹腔巨噬细胞分泌TNF-α·NO的影响

目的]研究金钗石斛多糖对脂多糖（LPS）诱导的小鼠腹腔巨噬细胞分泌肿瘤坏死因子-α（TNF-α）、一氧化氯（NO）的影响。方法]用不同浓度金钗石斛多糖作用于小鼠腹腔巨噬细胞，LPS作为诱导剂，采用放射免疫法检测细胞培养液中TNF—α的含量，硝酸酶还原法测定细胞培养液中NO的含量，荧光法测定细胞内诱导型一氧化氮合酶（iNOS）的活性，实时PCR（real—time PCR）法测定TNF-α mRNA、iNOS mRNA的表达。结果]与LPS组比较，金钗石斛多糖在50～400mg／L范围内可干预LPS对小鼠巨噬细胞的作用，使小鼠腹腔巨噬细胞TNF—α、NO合成减少，iNOS活性降低。TNF-α mRNA、iNOS mRNA的表达降低。结论]金钗石斛多糖可改善LPS对小鼠腹腔巨噬细胞的作用，使TNF—α mRNA、iNOS mRNA的表达降低，TNF—α、NO合成减少，从而起到抗炎的作用、

Effects of Polysaccharides from Dendribum nobile on TNF-α and NO Secretion in Peritoneal Macrophages of Mice Induced by LPS

Objective]Study on the effects of Dendribum nobile polysaccharide on tumor necrosis factor-α(TNF-α) and nitric oxide(NO) release by mouse peritoneal macrophage induced Lipopolysaccharide(LPS) in vitro.Method]The peritoneal macrophages of mice were conducted with LPS in the presence of Dendribum nobile polysaccharide at various concentration.TNF-αwas detected by radioimmunoassay,NO content of cell culture fluid was determined by nitrate reductase method,the activity of inducible nitric oxide synthase(iNOS) was measured by fluorimetry assay.The expression of TNF-αand iNOS mRNA were observed by real-time polymerase chain reaction(real-time PCR),Result]Compared with the control group,Polysaccharides of Dendribum nobile could markedly inhibit the secretion of TNF-αand NO and decrease the activity of iNOS and down-regulate the expression of TNF-αm RNA and iNOS mRNA in a dose dependent manner.Conclusion]Dendribum nobile polysaccharide can improve TNF-αmRNA、iNOS mRNA and its protein expression induced by Lipopolysaccharide in mouse peritoneal macrophage that may play a very important role in the prevention of inflammation.