| 二次回归正交设计优化紫茎泽兰中黄酮的提取工艺 |
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| 引用本文： | 杨郭,颜杰,马燮,彭健,杨强强.二次回归正交设计优化紫茎泽兰中黄酮的提取工艺[J].安徽农业科学,2009,37(3):917-919. |
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| 作者姓名： | 杨郭 颜杰 马燮 彭健 杨强强 |
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| 作者单位： | 四川理工学院,四川自贡,643000;四川理工学院,四川自贡,643000;四川理工学院,四川自贡,643000;四川理工学院,四川自贡,643000;四川理工学院,四川自贡,643000 |
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| 基金项目： | 四川理工学院大学生创新基金 |
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| 摘 要： | 目的]研究从紫茎泽兰中提取黄酮的最佳工艺条件。方法]以黄酮的得率为目标，使用单因素试验方法考察乙醇浓度、提取温度、固液比以及提取时间对黄酮得率的影响，使用回归正交组合设计优化提取工艺。结果]最优的黄酮工艺条件为：固液比为1：30，提取时间为2．5h，乙醇浓度为53％，提取温度为70℃。结论]使用回归正交组合设计能合理地优化紫茎泽兰中黄酮的提取工艺，为紫茎泽兰的综合利用提供参考。
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| 关 键 词： | 紫茎泽兰 黄酮 二次回归正交设计 |
Optimization of the Flavonoids Extraction from the Eupatorium adenophorum Spreng Using the Quadratic Orthogonal Regression Design |
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| Institution: | YANG Guo et al(Sichuan University of Science and Engineering,Zigong, Sichuan 643000) |
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| Abstract: | Objective] The aim of this study was to find the optimal extraction condition for the Eupatorium adenophorum spreng.Method] With the aim of extraction ratio of flavonoids,the single-factor experimental method was adopted to study on the influence such as mass concentration of ethanol,extraction temperature,solid-liquid ratio and extraction time,and the quadratic orthogonal regression rotational combinational design was used to optimize the extraction technique.Result] The optimal condition is as follows: the solid to liquid ratio is 1∶30,the extraction time is 2.5 hour,the mass contraction of ethanol is 53% and the extraction temperature is 70 ℃.Conclusion] The experimental method can well optimize the technique and it also provides an appropriate way to synthetically utilize the Eupatorium adenophorum spreng. |
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| Keywords: | Eupatorium adenophorum spreng Flavonoids Quadratic orthogonal regression design |
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