| 黄曲霉毒素B_1单克隆抗体修饰金电极检测饲料中的黄曲霉毒素B_1 |
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| 引用本文： | 孙玉雪,方俊杰,叶永康.黄曲霉毒素B_1单克隆抗体修饰金电极检测饲料中的黄曲霉毒素B_1[J].安徽农业科学,2013,41(10):4613-4614. |
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| 作者姓名： | 孙玉雪 方俊杰 叶永康 |
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| 作者单位： | 合肥工业大学生物与食品工程学院,安徽合肥,230009 |
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| 基金项目： | 教育部留学回国人员科研启动基金项目 |
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| 摘 要： | 目的]建立使用黄曲霉毒素B1单克隆抗体修饰金电极测定饲料中黄曲霉毒素B1的新方法。方法]试验采用电化学方法优化检测条件,建立标准曲线并对样品进行检测。结果]试验得出,黄曲霉毒素B1单克隆抗体修饰金电极检测饲料中黄曲霉毒素B1的最优条件为:电极修饰条件为4℃、8 h,磷酸盐缓冲溶液的pH为7.4。该检测方法对黄曲霉毒素B1的线性检测范围为1.427～150.000ng/ml,加标回收率在94.44%～101.36%。结论]使用该免疫电化学方法,检出限较低,可以用于饲料中黄曲霉毒素的检测。
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| 关 键 词： | 黄曲霉毒素B1单克隆抗体 修饰电极 黄曲霉毒素B1 饲料 |
Determination of Aflatoxin B1 in Feed Based on aAFB1 Modified Au Electrode |
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| Abstract: | Objective] A new method for determining the AFB1 in feed was established based on aAFB1 Modified Au Electrode.Method] The optimal conditions,calibration curve and determination of Aflatoxin B1 in feed all were got by electrochemical methods.Result] The optimal conditions: the Au electrode should be modified by 4 ℃,8 h,the optimal value of PBS is 7.4.Under the optimized conditions,the linear range is 1.427-150.000 ng/ml,the recovery rate is from 94.44% to 101.36%.Conclusion] The immunoelectrochemical method has a lower detection and can be applied to determination of AFB1 in feed successfully. |
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| Keywords: | Antibody-AFB1 Modified electrode Aflatoxin B1 Feed |
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