冷藏温度下凡纳滨对虾组织黑变与内源多酚氧化酶活性相关性研究

目的]探索凡纳滨对虾在冷藏过程中多酚氧化酶(PPO)活性变化程度与对虾黑变之间的相关性,明确关键控制点。方法]通过感官鉴定、光电色差和分光光度测定凡纳滨对虾冷藏过程中的黑变规律,据此研究PPO活性变化与凡纳滨对虾黑变之间的相关性。结果]试验表明,同一时间内凡纳滨对虾不同组织部位的PPO活性有明显差异,头部最高,腹部次之,最后是尾部。相关性分析表明,在冷藏过程中凡纳滨对虾黑变度与PPO活性变化均趋于上升,黑变度的变化规律与PPO的活性变化趋势呈显著正相关,头部、腹部、尾部的相关系数分别达到0.987 3、0.971 6、0.973 2,定量证明PPO活性变化是引起凡纳滨对虾黑变的主要因素。结论]研究可为凡纳滨对虾冷藏期间的黑变控制提供理论依据。

Correlation of Endogenesis Polyphenoloxidase Activity with Melanosis Accumulation in Litopenaeus vannamei during Cold Storage

Objective] To explore the correlation between PPO activity variation and melanosis of Litopenaeus vannamei during cold storage.Method] Melanosis of Litopenaeus vannamei during cold storage process was quantitatively studied by sensory analysis and color photoelectric measurement,and its correlation with polyphenoloxidase activity was assessed.Result] The results showed that there was a significant difference in polyphenoloxidase activity from different parts of Litopenaeus vannamei at the same time.The order was the head,abdomen and tail,respectively.The accumulation of melanosis presented a marked positive correlation with PPO activity and the correlation coefficients between melanosis and PPO activity in head,abdomen and tail were 0.955 2,0.9048 and 0.994 3,respectively.It can be concluded that the activation of PPO is the main reason of melanosis accumulation of Litopenaeus vannamei during cold storage process.Conclusion] The study can provide theoretical basis for melanosis control of Litopenaeus vannamei during cold storage.