地黄肌动蛋白基因片段的克隆与序列分析

目的]克隆与分析地黄肌动蛋白基因片段。方法]根据与地黄相近物种肌动蛋白基因序列的保守区设计兼并引物,通过RT-PCR方法扩增地黄编码区肌动蛋白基因。结果]扩增片段全长为724 bp,对应编码240个氨基酸。序列比对分析发现其与其他高等植物肌动蛋白基因核苷酸序列同源性在80%以上,氨基酸序列在90%以上。结论]系统进化分析表明,扩增到的地黄肌动蛋白基因与烟草actin7基因有较近的亲缘关系。

Cloning and Sequence Analysis of Actin Gene from Rehmannia glutinosa

Objective] The aim of this study is to clone and analyze the actin gene from Rehmannia glutinosa.Method] Degenerate primers were designed according to the conserved regions of actin sequences of Rehmannia glutinosa and its similar species,RT-PCR was next conducted to amplify the actin gene from Rehmannia glutinosa.Result] The amplified fragment is 724 bp and correspondingly 240 amino acids.The BLAST results indicate that the homology between the amplified fragment and other higher plants for actin gene sequences and amino acid are more than 80% and 90%,respectively,suggesting that the amplified fragment is the actin gene of Rehmannia glutinosa.Conclusion] Phylogenetic analysis shows that the actin gene of Rehmannia glutinosa has an intimate genetic relationship with actin7 gene of Nicotiana tabacum.