| 液质联用测定饲料中的β-兴奋剂 |
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| 引用本文： | 刘彩云,周围,毕阳,魏晋梅.液质联用测定饲料中的β-兴奋剂[J].安徽农业科学,2007,35(17):5178-5179. |
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| 作者姓名： | 刘彩云 周围 毕阳 魏晋梅 |
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| 作者单位： | 1. 甘肃农业大学食品科学与工程学院,甘肃兰州,730070
2. 甘肃省出入境检验检疫局,甘肃兰州,730070 |
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| 摘 要： | 对饲料中盐酸克伦特罗和莱克多巴胺的液质联用检测方法进行了研究。采用0.5%钨酸∶甲醇（80∶20）提取试样中的盐酸克伦特罗和莱克多巴胺,用Oasis MCX小柱净化,4 mmol/L乙酸胺溶液∶乙腈（80∶20）为流动相,用PDA检测器分离测定。盐酸克伦特罗和莱克多巴胺的检测限分别为1.05μg/kg和1.37μg/kg,平均回收率为89.7%和84.4%。该方法操作简单、结果准确,可用于测定饲料中盐酸克伦特罗和莱克多巴胺的含量。
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| 关 键 词： | 盐酸克伦特罗 莱克多巴胺 饲料 液质联用仪 |
| 文章编号： | 0517-6611（2007）17-05178-02 |
| 修稿时间： | 2007-03-07 |
Detection of β-agonist in Feedstuff by LC/MS |
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| LIU Cai-yun et al.Detection of β-agonist in Feedstuff by LC/MS[J].Journal of Anhui Agricultural Sciences,2007,35(17):5178-5179. |
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| Authors: | LIU Cai-yun |
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| Institution: | College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, Gansu 730070 |
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| Abstract: | Detection of clenbuterol and ractopamine in feedstuff was studied by liquid chromatography mass spectrometry(LC/MS).The clenbuterol and ractopamine of specimen was extracted by 0.5 % tungstenic acid: methanol(80∶20).It was then purified by Oasis MCX.The mobile phase was 4 mmol/L ammonium acetate∶acetonitrile(80∶20).PDA detector was used for separation and detection.Detection limits of clenbuterol and ractopamine were 1.05 μg/kg and 1.37 μg/kg and the average rates of their recovery were 89.7 % and 84.4 %,respectively.The method was simple and precise,which could be used for the detection of clenbuterol and ractopamine in feedstuff. |
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| Keywords: | Clenbuterol Ractopamine Feedstuff LC/MS |
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