西藏山羊DQA2基因多态性与血液免疫性状的相关性分析

目的]了解西藏山羊DQA2基因与血液免疫性状的关系。方法]利用PCR-RFLP对西藏山羊DQA2基因外显子2多态性及其血液免疫指标效应进行了分析。结果]在西藏山羊中共检测到4种基因型,由3个等位基因控制。其中,BB基因型淋巴细胞数目显著高于AB型和BC型(P0.05),BB型中间细胞数目高于AB型和BC型,BB型平均血红蛋白含量低于AB型和BC型,但均差异不显著。结论]西藏山羊BB基因型是影响血液淋巴细胞总数的重要因素,与血液免疫指标有一定的相关性,可作为抗病育种的遗传标记,也为后期的辅助选择提供了科学依据。

Correlation Analysis between Polymorphisms of DQA2 Gene and Blood Immune Traits in Tibetan Goats

Objective] To understand the relationship between DQA2 gene and blood immune traits in Tibet goat.Method]The polymorphism of exon 2 of DQA2 gene of Tibetan goat and the effects on blood immune traits were studied by PCR-RFLP. Result] Four genotypes were detected in Tibetan goat,which were controlled by three alleles.Lymphocyte count(W-SCC)with genotype BB was significantly higher than that with genotype AB and BC (P<0.05).Intermediate cell count (MID) with genotype BB was higher than that with genotype AB and BC.Mean corpuscular he-moglobin(MCH) with genotype BB was lower than that with genotype AB and BC,but there was no significant difference.Conclusion]Genotype BB was an important factor affecting W-SCC.There was correlation between genotypes and blood immune traits,so it could be used as a genetic marker for disease resistance breeding,and it also provided scientific basis for the latter selective breeding.