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防治西瓜枯萎病的微生物菌种的筛选与鉴定
引用本文:陈允亮,毛慧渊,陶黎明.防治西瓜枯萎病的微生物菌种的筛选与鉴定[J].安徽农业科学,2017,45(28).
作者姓名:陈允亮  毛慧渊  陶黎明
作者单位:复旦大学化学系,上海200433;镇江恒欣生物科技有限公司,江苏镇江212100;镇江恒欣生物科技有限公司,江苏镇江,212100;华东理工大学药学院,上海,200237
基金项目:江苏省发改委重大环境治理工程2014年中央预算内投资计划,2014年镇江市农业科技支撑项目,2015年镇江市丹徒区农业科技项目
摘    要:目的]对一组防治西瓜枯萎病的微生物菌种进行鉴定。方法]以西瓜专化型尖孢镰刀菌为研究对象,从海洋海绵共生菌和土壤样品中筛选到6株具显著拮抗西瓜专化型尖孢镰刀菌的活性菌株,并对该6株活性菌株进行分子鉴定。结果]分子鉴定显示该6株活性菌株均属于芽孢杆菌属的物种。结论]试验结果为西瓜枯萎病类安全高效的生物农药及功能型生物肥料的创制提供了理论依据。

关 键 词:枯萎病  生物防治  尖孢镰刀菌  芽孢杆菌

Screening and Identification of Microorganism against Watermelon Fusarium Wilt
CHEN Yun-liang,MAO Hui-yuan,TAO Li-ming.Screening and Identification of Microorganism against Watermelon Fusarium Wilt[J].Journal of Anhui Agricultural Sciences,2017,45(28).
Authors:CHEN Yun-liang  MAO Hui-yuan  TAO Li-ming
Abstract:Objective] The aim was to identify microorganism against watermelon Fusarium wilt.Method] The Fusarium oxysporum f.sp. niveum was taken as the research object and six strains with significant antagonistic activity to Fusarium oxysporum f.sp.niveum from symbiotic bacteria of marine sponges and soil samples were screened, and the six active strains was identified through molecular biological characteriza-tion. Result] The molecular biological characterization showed that these six active strains all belonged to Bacillus. Conclusion] The conclu-sion of this study lays a foundation for new type of safe and efficient biological pesticide and functional biological fertilizer towards watermelon wilt.
Keywords:Wilt  Biological control  Fusarium oxysporum  Bacillus
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