| 产纤维素酶绿色木霉F-UV_(264)产酶条件优化 |
| |
| 引用本文： | 林英,秦萍,杜志强,张蓓蓓.产纤维素酶绿色木霉F-UV_(264)产酶条件优化[J].安徽农业科学,2006,34(11):2312-2314. |
| |
| 作者姓名： | 林英 秦萍 杜志强 张蓓蓓 |
| |
| 作者单位： | 沈阳农业大学生物科学技术学院,辽宁,沈阳,110161 |
| |
| 摘 要： | 对诱变菌株绿色木霉F-UV264产纤维素酶条件进行了研究,结果表明:以1%(NH4)2SO4和2%酵母膏为氮源,并加入3%表面活性剂,2%Mandels营养盐,麸皮与稻草粉比例为1∶3,加水量为干料的4倍,可提高酶的产量。该菌株最适产酶培养条件为pH值4.6~5.6,30℃条件下发酵96~135 h纤维素酶的酶活性达到较高水平。此研究可为工业化生产纤维素酶提供依据。
|
| 关 键 词： | 纤维素酶 绿色木霉 产酶条件 |
| 文章编号： | 0517-6611（2006）11-2312-02 |
| 收稿时间： | 2006-04-10 |
| 修稿时间： | 2006-04-10 |
study on Optimization Producing Cellulase Conditions of Trichoderma |
| |
| LIN ying et al.study on Optimization Producing Cellulase Conditions of Trichoderma[J].Journal of Anhui Agricultural Sciences,2006,34(11):2312-2314. |
| |
| Authors: | LIN ying |
| |
| Institution: | Shengyang Agdcultural University,Shenyang,Liaoning 110161 |
| |
| Abstract: | In this paper,the producing cellulase conditions of mutant stain Trichoderma F-UV_(264) were stduied.The result of experiment showed that: the optimal nitrogen source is 1%(NH_4)_2SO_4 and 3%the surface active material,the optimal sult group is 2%Mandels,bran:straw is 1∶3,solid:liquid is(1∶4).The enzyme vitality of cellulase can be increased after adding these influental factors.The optimum culture condition of producing cellulase is pH(4.6)～5.6 at 30 ℃ for 96～135 h,and the maximum cellulase activity is reached.This research can provide the basis for the cellulase manufacture. |
| |
| Keywords: | Cellulase activity Trichoderma Producing cellulase conditions |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
