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| 高原鼠兔HIF-1α基因真核表达载体的构建及细胞表达和定位 | |
| 引用本文： | 李红阁,赵新全,常智杰,任永明,王德鹏.高原鼠兔HIF-1α基因真核表达载体的构建及细胞表达和定位[J].安徽农业科学,2009,37(10):4386-4388. |
| 作者姓名： | 李红阁 赵新全 常智杰 任永明 王德鹏 |
| 作者单位： | 1. 中国科学院西北高原生物研究所,高原生物适应与进货重点实验室,青海西宁,810001;中国科学院研究生院,北京,100049 2. 中国科学院西北高原生物研究所,高原生物适应与进货重点实验室,青海西宁,810001 3. 清华大学医学院生物科学与技术系,生物膜与膜生物工程国家重点实验室,北京,100084 |
| 基金项目： | 国家基础研究规划项目(2005CB422005) |
| 摘 要： | 利用PCR从克隆质粒PMD18-T-pHIF-1α中扩增鼠兔HIF-1α;构建重组质粒pcDNA3.0/6Xmyc-p HIF-1α,用重组质粒转染HEK-293T,HeLa和COS-7细胞,通过免疫印迹分析,检测HIF-1α表达蛋白在细胞中的分布。EcoRI和XhoⅠ酶切鉴定和序列分析表明,构建的pcDNA3.0/6Xmyc-pHIF-1α真核表达载体序列正确;HIF-1α在真核细胞中能够正确表达,且其表达产物主要存在于细胞核中。 |
| 关 键 词： | 高原鼠兔 HIF-1α基因 真核表达载体 |
Construction of Eukaryotic Expression Vector of HIF-1α Gene in Plateau Pika and Its Expression and Localization in Cell |
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| LI Hong-ge et al.Construction of Eukaryotic Expression Vector of HIF-1α Gene in Plateau Pika and Its Expression and Localization in Cell[J].Journal of Anhui Agricultural Sciences,2009,37(10):4386-4388. | |
| Authors: | LI Hong-ge |
| Institution: | LI Hong-ge et al(Key Laboratory of Adaptation , Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,Qinghai 810001) |
| Abstract: | HIF-1α in cloning plasmid PMD18-T-pHIF-1α was amplified by PCR,and the recombinant plasmid pcDNA3.0/6Xmyc-pHIF-1α was constructed and was transfected into HEK-293T,Hela and COS-7 cells.The distribution of the protein expressed by HIF-1α in cells was detected by western blot analysis.The results of enzyme digestion and sequence analysis showed that the sequence of the constructed eukaryotic expression vector was correct.The HIF-1α gene could correctly expressed in eukaryotic cells and its expression products mainly exist in cell nuclear. |
| Keywords: | Plateau pika HIF-1α gene Eukaryotic expression vector |
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