复合酶法提取南瓜多糖及其抗氧化性的研究

目的]优化复合酶法提取南瓜多糖的工艺条件,研究南瓜多糖的抗氧化性。方法]采用单因素试验设计研究了不同提取时间、温度、料液比、pH值对南瓜多糖提取率的影响,并通过正交试验确定了提取南瓜多糖的最佳复合酶配比和最佳提取条件。采用水杨酸法检测南瓜多糖对羟基自由基（.OH）和改进的邻苯酚自氧化法检测其对超氧阴离子自由基（O-2）的清除效果。结果]当纤维素酶的浓度为1.0%、果胶酶为1.5%、木瓜蛋白酶为1.0%时,以及温度为40℃、pH=4.6、料液比为1∶30、提取时间为30 m in的条件下南瓜多糖的提取率最高;南瓜多糖对.OH具有较好的清除效果,对O-2有部分清除作用。结论]该研究为南瓜多糖的研究及应用提供了基础资料。

Extraction of Pumpkin Polysaccharide by Complex Enzyme Method and Its Antioxidant Research

Objective] This paper aims to study a new method of extracting pumpkin polysaccharide from pumpkin.Single factor experiments were conducted to examine the effects of extracting time,temperature,the solid-liquid ratio and pH value on the extraction yield of polysaccharide from pumpkin.Method] The best enzyme ratio and extraction conditions for complex enzymes extraction were determined through orthogonal tests.Scavenging ·OH and O-2 activities of pumpkin polysaccharides were also investigated by salicylic acid and improved self-oxidation of o-pheno methods respectively.Result] The results showed that the biggest extraction yield of polysaccharide from pumpkin can be got when adding 1% cellulose enzyme,1.5% pectinase,1.0% papain and Na2HPO4-citric acid buffer solution(pH was 4.6),and oscillating for 30 min under water at 40 ℃ with the solid-liquid ratio of 1∶30.In addition,pumpkin polysaccharides had a strong activity of eliminating oOH,but very weak activity to scavenge O-2.Conclusion] This study provided basic data for research and application of Pumpkin polysaccharide.