红菇娘和紫菇娘的组织培养与繁殖

目的]筛选野生红菇娘(Calyx seu Fructus Physalis)和紫菇娘(Physalis tungkenensis Kuan et Gao)组织培养的最佳培养基。方法]分别以2种植物的茎、叶为外植体,比较不同取样方式、不同增殖条件及不同生根条件对2种植物组织培养和快速繁殖的影响。结果]以MS+6-BA 3.0 mg/L+NAA 0.04 mg/L为最适合红菇娘愈伤组织诱导和分化的培养基,而紫菇娘是MS+6-BA 3.0 mg/L和NAA0.03 mg/L,培养14 d后每个愈伤组织能产生3~5个不定芽。继代和生根培养后,丛生芽的成活率为90%以上。结论]该研究可为2种酸浆属野生种质资源保存和优质种苗生产提供技术参考。

Tissue Culture and Reproduction of Calyx seu Fructus Physalis and Physalis tungkenensis Kuan et Gao

Objective] The best tissue culture medium for Calyx seu Fructus Physalis and Physalis tungkenensis Kuan et G ao was selected.Method]Stem and leaf were used as the explants for the tissue culture and rapid propagation by comparing different sampling methods,different breeding conditions and different rooting conditions.Result] MS + 6-BA 3.0 mg/L + NAA 0.04 mg/L was the best medium for callus induction and bud differentiation of Calyx seu Fructus Physalis,MS + 6-BA3.0 mg/L + NAA 0.03 mg/L was the best medium for callus induction and bud differentiation of Physalis tungkenensis Kuan et Gao,there were 3 and 5 clustered shoots per one callus induction for both Calyx seu Fructus Physalis and Physalis tungkenensis Kuan et Gao respectively after 14 days.The survival rate reached 90％ after subculturing and rooting culture.Conclusion] The study can provide technical reference for the preservation of wild germplasm resources and the production of high-quality seedlings.