| 一种适宜拟南芥PCR检测的DNA提取方法 |
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| 引用本文： | 徐平丽,赵晋平,孟静静,李保龙,李新国,郭峰.一种适宜拟南芥PCR检测的DNA提取方法[J].安徽农业科学,2010,38(13):6653-6654. |
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| 作者姓名： | 徐平丽 赵晋平 孟静静 李保龙 李新国 郭峰 |
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| 作者单位： | 山东省农业科学院高新技术研究中心,山东省作物与畜禽品种改良生物技术重点开放实验室,山东济南,250100;山东省曹县种子公司,山东菏泽,274400 |
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| 基金项目： | 国家科技支撑计划，山东省优秀中青年科学家研究奖励基金，山东省农业科学院青年基金 |
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| 摘 要： | 目的]介绍一种适于拟南芥PCR检测的DNA快速提取方法。方法]通过对常规DNA提取方法的改进,获得可以快速大批量地提取拟南芥DNA样品的方法,并以随机抽取的拟南芥转基因株系和突变株系为样品进行验证。结果]经过琼脂糖凝胶电泳及紫外吸收检测,DNA样品完整且污染少,PCR扩增目的片段结果良好,适于作为PCR反应的模板。经过对随机抽取的拟南芥转基因株系和突变株系的PCR检测,阳性植株目的基因扩增条带清晰,无假阳性,试验结果理想。结论]该方法适用于拟南芥DNA样品的快速提取、PCR检测及拟南芥突变体筛选工作。
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| 关 键 词： | 拟南芥 PCR DNA快速提取 |
A Rapid DNA Extraction Method for PCR Detection of Arabidopsis thaliana |
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| Institution: | XU Ping-li et al ( Key Laboratory of Improved Biotechnology of Crops and Livestock and Poultry in Shandong Province, High-tech Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100) |
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| Abstract: | Objective] The aim was to introduce a rapid DNA extraction method for PCR detection of Arabidopsis thaliana. Method] Through the improvement of conventional DNA extraction method,a rapid Arabidopsis thaliana DNA extraction method was obtained. With randomly selected Arabidopsis thaliana transgenic strains and mutants as samples,the method was verified. Result] After electrophoresis,UV absorption detection,it was found that DNA samples are complete and less pollution,and the result of PCR amplification objective fragment was good which proved DNA is suitable as a template for PCR reaction. After PCR detection,positive plants gene amplified bands were clear,without false-positive,and the test results were satisfactory. Conclusion] The method is suitable for rapid extraction of Arabidopsis thaliana DNA、PCR detection and screening of Arabidopsis thaliana. |
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| Keywords: | PCR |
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