| 霍山石斛组培快繁技术研究 |
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| 引用本文： | 傅玉兰,谷凤,胡传明,杨海燕,黄新.霍山石斛组培快繁技术研究[J].安徽农业科学,2004,32(3):522-523. |
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| 作者姓名： | 傅玉兰 谷凤 胡传明 杨海燕 黄新 |
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| 作者单位： | 安徽农业大学森林利用学院,安徽,合肥,230036 |
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| 摘 要： | 为提高霍山石斛的繁殖系数 ,对霍山石斛试管播种苗组培快繁技术进行了试验研究。结果表明 :不同的植物激素及其浓度组合、培养基中糖的含量、pH值等因素对霍山石斛试管苗的生长及继代增殖有很大的影响。其中 ,以MS为基本培养基 ,添加植物激素KT 3mg/L、2 ,4-D 0 .2mg/L、糖 3 % ,pH值 5 .4,对促进试管苗生长和继代增殖的效果最佳 ;而试管苗出瓶移栽的最佳基质为树皮块 ,其次为椰壳糠。
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| 关 键 词： | 霍山石斛 组织培养 植物激素 试管苗 快速繁殖 |
| 文章编号： | 0517-6611(2004)03-0522-02 |
Research on the Tissue Culture and Rapid Propagation of Dendrobium Huoshanense |
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| Fu Yulan et al.Research on the Tissue Culture and Rapid Propagation of Dendrobium Huoshanense[J].Journal of Anhui Agricultural Sciences,2004,32(3):522-523. |
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| Authors: | Fu Yulan |
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| Abstract: | In order to improve propagation rate of Dendrobium Huoshanense, the experiment in micro-propagation technology of tube seedling in Dendrobium Huoshanense was conducted. The results showed: different plant growth regulators and concentration combination, different sugar amounts and pH values had obverious effect on tube seedling growing and propagation. Among those, the best combination was MS+KT*!3*! mg/L +2.4-D*!0.2*!mg/L + sugar 3% with pH=5.4.The best transplanting medium was cortex.The better was coconut bran. |
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| Keywords: | Dendrobium huoshanense Tissue culture Plant growth regulator Tube seedling Rapid propagation |
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