柳枝稷的组织培养技术研究

目的]探索柳枝稷的不同组培条件，优化其诱导和分化培养基。方法]以柳枝稷幼穗为外植体进行组织培养获得组培苗，建立相应的植株再生系统。结果]愈伤组织诱导培养基为MS＋5．00mg／L2，4-D＋0．15mg／L6．BA＋3．00％蔗糖＋0．75％琼脂；继代与增殖培养基为MS＋4．00mg／L2，4-D＋3．00％蔗糖＋0．75％琼脂；分化培养基为MS＋0．2mg／L KT＋3．00％蔗糖＋0．75％琼脂；生根培养基为1／2MS＋0．80％蔗糖＋0．70％琼脂。愈伤组织诱导和继代培养阶段培养温度为24℃，在分化和生根培养阶段培养温度为28℃。幼穗外植体的出愈率达95％，愈伤组织增殖率在800％-1000％以上，分化率达80％以上，生根率在98％以上。经炼苗后，获得的组培苗的移栽成活率达95％以上。结论]采用优化激素搭配的培养基可得到高效的诱导率、分化率和生根率。

Brief Report on Tissue Culture of Panicum virgatum

Objective] The study aimed to explore different tissue culture conditions of Panicum virgatum and optimize its induction and differentiation medium.Method] With the young spike of P.virgatum as explant,the tissue cultured seedlings were obtained and the corresponding plant regeneration system was established.Result] The medium for inducing callus was MS+ 5.00 mg/L 2,4-D+0.15 mg/L 6-BA +3.00% sucrose+0.75% agar,that for subculture and proliferation was MS+4.00 mg/L 2,4-D +3.00% sucrose +0.75% agar,that for differentiation was MS+0.20 mg/L KT +3.00% sucrose +0.75% agar and that for rooting was 1/2MS+0.80% sucrose+0.70% agar.The culture temperature at the stage of the callus induction and subculture was 24 ℃ and that at the culture stage of the differentiation and rooting was 28 ℃.The callus induction rate of the explant of young spike was 95%,the proliferation rate of callus was above 800%-1 000%,the differentiation rate was above 80% and the rooting rate was above 98%.The transplanting survival rate of obtained tissue cultured seedlings was above 95% after training seedling.Conclusion] The high-effective induction rate,differentiation rate and rooting rate could be obtained by using the medium with optimizing hormone combination.