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棉花黄萎病菌菌丝生长和产孢量的影响因素研究
引用本文:纪文飞,高智谋,曹君,邹艳,杜雷,陈伟.棉花黄萎病菌菌丝生长和产孢量的影响因素研究[J].安徽农业科学,2006,34(17):4330-4331.
作者姓名:纪文飞  高智谋  曹君  邹艳  杜雷  陈伟
作者单位:安徽农业大学植保学院,安徽,合肥,230036
基金项目:安徽省高校科拔尖人才基金 , 安徽省科技攻关项目
摘    要:研究不同温度、pH值以及碳源、氮源对棉花黄萎病菌菌丝生长速率和分生孢子产生量的影响。结果表明,在0~33℃下,供试菌株均能生长,最适温度为25℃;在pH值为3~10时均能生长,最适pH值为5~8;葡萄糖作为碳源时,该病菌在培养基平板上生长最快,分生孢子的产生量最大;氮源以硝酸钠为最佳,其次为脲。硫酸铵作为氮源时菌丝生长速率低于无氮源对照,菌落不形成微菌核,产孢量小,这说明硫酸铵为氮源时不利于病菌菌丝生长及微菌核和分生孢子的形成。

关 键 词:棉花黄萎病菌  菌丝生长速率  产孢量
文章编号:0517-6611(2006)17-4330-02
收稿时间:05 15 2006 12:00AM
修稿时间:2006-05-15

Effect of Temperature, pH and Nutrient on the Mycelia Growth and Conidial Production of Verticillium dahliae
JI Wen-fei et al.Effect of Temperature, pH and Nutrient on the Mycelia Growth and Conidial Production of Verticillium dahliae[J].Journal of Anhui Agricultural Sciences,2006,34(17):4330-4331.
Authors:JI Wen-fei
Institution:College of Plant Protection, Anhui Agricultural University, Hefei, Anhui 231X
Abstract:The effects of temperature,pH,carbon and nitrogen sources on the mycelium growth and conidia production of Verticillium dahliae Kled and the pathogenic fungus causing Verticillium wilt of cotton in Anhui were studied respectively.The results showed that for the mycelium growth of Verticillium dahliae,the range of temperature was from 0?℃ to 33?℃,with the optimum being 25?℃ and the range of pH was from 3 to 10,with the optimum being 5 to 8.Glucose was the best carbon source for the mycelium growth of the fungus.Among the tested nitrogen sources,sodium nitrate was the best,next was urea.Ammonium sulfate was inhibitory to the mycelium growth,micro-sclerotium formation and conidia production of the pathogenic fungus.
Keywords:Verticillium dahliae  Mycelia growth rate  Conidial production
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