均匀设计法优选大黄酸提取工艺研究

目的]探讨提取相关因素与提取物中大黄酸含量的关系,研究大黄中大黄酸的最佳提取工艺。方法]采用均匀设计法探讨以乙醇水为溶剂时,使用TS-NS系列小试型提取浓缩装置以粉碎度、乙醇浓度、固液比、提取时间为探讨因素,以大黄酸含量作为考察指标,来筛选大黄酸的最佳工艺研究。结果]大黄酸优选工艺为85%乙醇作为提取溶剂,大黄颗粒过18目筛,固液比为1∶10,提取时间为0.5 h。结论]该工艺科学、合理,可为以乙醇水为溶剂提取大黄中大黄酸的工艺优选提供科学依据。

Study on the Extraction Process of Rhein from RADIX ET RHIZOMA RHEI by Uniform Design

Objective] To discuss the relationship between related factors and rhein content and optimize the extraction process of rhein from RADIX ET RHIZOMA RHEI by uniform design method.Method] Taking the content of rhein as index,uniform design with 4 investigated factors including comminution degree,ethanol concentration,solid-liquid ratio,and extraction time was performed to optimize the extraction process of rhein from RADIX ET RHIZOMA RHEI with TS-NS extraction and concentration device.Result] The extraction rate of rhein was the highest in extracts when RADIX ET RHIZOMA RHEI powder sieved with 18 meshes was extracted for 0.5 h with 85% ethanol as solvent under solid-liquid ratio of 1∶10.Conclusion] The technology was scientific and reasonable that could provide a scientific basis for optimizing the extraction process of rhein from RADIX ET RHIZOMA RHEI using ethanol as extracting solvent.