| 抗稻瘟病基因Pib4的转基因研究 |
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| 引用本文： | 刘文平,温嘉伟,林秀峰,林春晶,王继春,王嘉琦,王海.抗稻瘟病基因Pib4的转基因研究[J].安徽农业科学,2011,39(22):13303-13305. |
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| 作者姓名： | 刘文平 温嘉伟 林秀峰 林春晶 王继春 王嘉琦 王海 |
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| 作者单位： | 吉林省农业科学院植物保护研究所,吉林公主岭,136100;吉林省农业科学院生物技术中心,吉林长春,130124;吉林省榆树市延和乡农业技术推广站,吉林榆树,130421;吉林省榆树市大岭镇农业技术推广总站,吉林榆树,130414 |
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| 基金项目： | 吉林省农业科学院引进人才项目;吉林省科技厅项目;吉林省财政厅项目;人社部对回国留学人员择优资助项目 |
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| 摘 要： | 目的]探讨抗稻瘟病基因Pib4的转基因。方法]利用PCR法克隆Pib基因的NBS功能结构域到双元载体pCAMBIA1301上,以粳稻品种农大18的成熟胚诱导出愈伤组织,以愈伤组织作为转化的受体,采用农杆菌介导法转化Pib4基因。结果]经PCR和GUS活性检测表明外源基因已整合到水稻基因组中并获得表达;用稻瘟病菌接种阳性植株,获得了抗稻瘟病菌的水稻植株。结论]为合理利用抗病基因和育种提供了参考。
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| 关 键 词： | 稻瘟病 抗病基因 Pib基因 |
Transgene of Rice Blast Resistance Gene Pib4 |
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| Institution: | LIU Wen-ping et al(Institute of Plant Protection,Jilin Academy of Agriculture Science,Gongzhuling,Jilin 136100) |
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| Abstract: | Objective] The aim was to discuss the transgene of rice blast resistance gene Pib4.Method] NBS domain of Pib4 gene was amplified by PCR and subcloned into the binary vector pCAMBIA1301,the Pib4 gene by Agrobacterium tumefaciens were transferred into calli which derived from mature embryos of japonica rice Nongda 18.Result] Transgenic rice was confirmed by PCR and GUS analysis.Positive transgenic plants were inoculated with Magnaperthe grisea fungus,which had the same resistance with control plant.Conclusion] All those provide references for using disease-resistant gene and breeding rationally. |
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| Keywords: | Rice blast Disease-resistant gene Pib gene |
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