银杏叶叶绿素荧光特性及其含量测定研究

目的]研究银杏叶叶绿素的荧光特征及其含量的变化规律。方法]以新鲜银杏叶为材料,采用混合有机溶剂进行萃取,并用分光光度法对银杏叶叶绿素的含量进行测定,通过比较不同月份银杏叶叶绿素的含量,得到叶绿素含量的变化规律,同时研究银杏叶叶绿素的特征荧光发射峰和磷光寿命。结果]银杏叶叶绿素有2个特征荧光发射峰,分别位于691和718 nm处,在2个发射峰处的磷光寿命分别为8.61和8.21μs;在不同月份的银杏叶中,叶绿素含量从大到小依次为7月、4月、11月,但叶绿素a/b比值从大到小依次为4月、11月、7月。结论]该研究为银杏叶叶绿素的进一步研究提供参考。

Study on Fluorescence Characteristics and Content of Chlorophyll in Ginkgo Leaves

Objective] The research aimed to study fluorescence characteristics and content variance of chlorophyll in ginkgo leaves.Method] Fresh ginkgo leaves were used as raw material,chlorophyll was extracted by mixed organic solvent and its content was measured with spectrophotometry.Chlorophyll contents in ginkgo leaves in different months were compared to obtain variance of chlorophyll content,and characteristic fluorescence peaks and phosphorescence lifetimes of chlorophyll were studied.Result] The chlorophyll of ginkgo leaves had two characteristic fluorescence peaks and they were located at 691 nm and 718 nm,and the phosphorescence lifetimes of chlorophyll of ginkgo leaves were 8.61 and 8.21 μs at two emission peaks respectively.The chlorophyll content in ginkgo leaves in July was the highest,and that in April ranked the second,and that in November was the lowest.However,the order of chlorophyll a/b value in different months was April ＞ November ＞ July.Conclusion]This study provides guidance for further research of chlorophyll in ginkgo leaves.