不同方法及发酵管数量对大肠菌群检测结果的差异性分析

目的]比较不同方法检测同种水样中大肠菌群含量的结果是否具有差异性,以及多管发酵法中发酵管数量对检测结果的影响。方法]分别采取污染程度由低至高的A、B、C 3种水样,通过多管发酵法、纸片法、滤膜法检测相同水样中大肠菌群的含量,并在多管发酵管法中利用9、10、15管发酵检测同种水样,采用SPSS软件对试验数据进行差异性分析。结果]多管发酵法与纸片法差异不显著,结果具有一致性,而滤膜法与其他2种方法获得结果具有显著性差异。对9、10、15管发酵检测3种水样获得的结果进行方差分析,得到的P值分别是0.709、0.964、0.840,均大于0.05,故差异不显著,结果具有一致性。结论]试验结果为大肠菌群检测方法的研究提供了理论依据。

Differences of Different Methods and Number of Fermentation Tubes on Coliform Test Results

Objective] The aim was to compare the difference between the results of different methods to detect the content of coliforms in the same water samples and the effect of the number of fermentation tubes in the multi-tube fermentation method on the detection results.Method] Three kinds of water samples of A,B and C with low pollution level were adopted,and the contents of coliform bacteria in the same water samples were detected by multi-tube fermentation,paper and filter method.The same kind of water samples were tested by 9,10,15 tube fermentation.The results were analyzed by SPSS software.Result] The difference between the multi-tube fermentation method and the paper method was not significant,the results were consistent,and the filter method was significantly different from the other two methods.P values were 0.709,0.964 and 0.840,respectively,which were more than 0.05,so the difference was not significant and the results were consistent.Conclusion] The results provide reference for the detection of coliform bacteria.