副溶血性弧菌的检测研究

目的]对toxR进行副溶血性弧菌特异性检测,探讨toxR靶基因能否准确检测副溶血性弧菌,从而消除其他研究者对该基因特异性的疑虑。方法]采用SN/T1870—2016中副溶血性弧菌toxR的引物探针对副溶血性弧菌和其亲缘关系接近的弧菌标准菌株进行检测。结果]采用实时荧光PCR方法证实该引物探针只能扩增出副溶血性弧菌,其他弧菌诸如溶藻弧菌、创伤弧菌、霍乱弧菌等未得到扩增。结论]证实SN/T1870—2016中toxR的引物探针特异性高。该研究可为各检测机构提供数据支持,为副溶血性弧菌的检测与研究奠定更为坚实的基础。

Detection Research of Vibrio parahaemolyticus

Objective] The specific detection of toxR in Vibrio parahaemolyticus was used to investigate whether the toxR target gene can detect Vibrio parahaemolyticus accurately,thus it eliminates doubts among other researchers about the specificity of the gene.Method] The primer and probe of toxR in SN/T 1870-2016 was used to detect Vibrio parahaemolyticus and its Vibrio related standard strain.Result] The real-time fluorescent PCR method proved that the primer and probe could only amplify Vibrio parahaemolyticus,and other Vibrio species such as Vibrio vulnificus,Vibrio vulnificus and Vibrio cholerae were not amplified.Conclusion] The specificity of toxR primers and probe in SN/T1870-2016 was confirmed high.This study can provide data support for the testing organization,and lay a more solid foundation for the detection and research of V.Parahaemolyticus.