| 山东省野生枸杞多糖的分离·纯化和组分分析 |
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| 引用本文： | 商希礼,魏永祥.山东省野生枸杞多糖的分离·纯化和组分分析[J].安徽农业科学,2010,38(24):13047-13048. |
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| 作者姓名： | 商希礼 魏永祥 |
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| 作者单位： | 滨州学院化学与化工系,山东滨州,256600;滨州学院化学与化工系,山东滨州,256600 |
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| 基金项目： | 滨州学院青年人才创新工程项目 |
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| 摘 要： | 目的]分离纯化山东省野生枸杞多糖（Lycium barbarum Polysaccharide,LBP）,并对其组分进行分析。方法]用水提法从枸杞中提取分离枸杞多糖,用DEAE纤维素柱和SephadeexG-100柱进行纯化,用苯酚-硫酸法测定活性多糖的含量,用红外光谱分析其组分。结果]测得山东野生枸杞中枸杞多糖的含量为12.56%。LBP含有4种组分LBP-Ⅰ、LBP-Ⅱ、LBP-Ⅲ和LBP-Ⅳ。结论]该研究可为今后更深入地研究枸杞多糖的结构特征和活性功能等提供依据。
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| 关 键 词： | 枸杞多糖 分离 纯化 组分分析 |
Isolation, Purification and Components Analysis of Polysaccharide from Lycium barbarum of Shandong Province |
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| Institution: | SHANG Xi-li et al (Department of Chemistry and Engineering,Binzhou University,Binzhou,Shandong 256600) |
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| Abstract: | Objective] The research aimed to isolate and purify polysaccharide from Lycium barbarum of Shandong Province,and analyze its components. Method] LBP was extracted from Lycium barbarum with water and purified with DEAE ion-exchange cellulose column and Sephadeex G-100 column,and the Lycium barbarum polysaccharide（LBP） content was determined with phenol-sulfuric acid method. Result] The results showed that LBP content was 12.56%. LBP had 4 water soluble polysaccharides,LBP-Ⅰ,LBP-Ⅱ,LBP-Ⅲ and LBP-Ⅳ. Conclusion] The study can provide basis for deeply researching the structural characteristics and function of Lycium barbarum. |
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| Keywords: | Lycium barbarum Polysaccharide （LBP） Extraction Purification Components analysis |
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