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| 紫花苜蓿根腐性病害生防放线菌的分离与筛选 | |
| 引用本文： | 王兰英,徐林波,何勇,乌兰巴特尔,赵海霞.紫花苜蓿根腐性病害生防放线菌的分离与筛选[J].安徽农业科学,2010,38(15):7944-7946. |
| 作者姓名： | 王兰英 徐林波 何勇 乌兰巴特尔 赵海霞 |
| 作者单位： | 1. 海南大学环境与植物保护学院,海南儋州,571737 2. 中国农业科学院草原研究所,内蒙古呼和浩特,010010 3. 青岛海源草坪有限公司,山东青岛,026300 |
| 基金项目： | 中央级公益性科研院所基本科研业务费基金专项 |
| 摘 要： | 目的]分离筛选出对苜蓿根腐病病原具有高拮抗作用的生防放线菌。方法]利用梯度稀释分离法从来自内蒙古赤峰地区的10个土样中分离得到91株放线菌。以3株苜蓿根腐病菌为靶标菌,采用平皿对峙培养法,对分得菌株进行皿内拮抗作用测定。结果]获得5株对供试苜蓿根腐病菌有较强抑制作用的拮抗菌株,编号为1-3-6、4-4-2、6-2-25、6-2-27、7-2-13,分别来自1号、4号、6号及7号土样,占分离菌株的5.50%。结论]该研究可为苜蓿根腐病生物防治奠定一定基础。 |
| 关 键 词： | 放线菌 拮抗作用 苜蓿 根腐病 |
Isolation and Screening of Alfalfa Root Rot Biocontrol Actinomyces |
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| WANG Lan-ying et al.Isolation and Screening of Alfalfa Root Rot Biocontrol Actinomyces[J].Journal of Anhui Agricultural Sciences,2010,38(15):7944-7946. | |
| Authors: | WANG Lan-ying |
| Institution: | WANG Lan-ying et al(College of Environmental and Plant Protection,Hainan University,Danzhou,Hainan 571737) |
| Abstract: | Objective]Actinomycetes with high antagonistic effects on alfalfa root rot pathogen was isolated from soil.Method]91 actinomyces were isolated from 10 soil samples in Chi feng Inner Mongolia by gradient dilution method.In duel culture tests,all isolates were tested for their antagonism by using 3 strains including Fusarium solani,F.Oxysporum,F.avenaceum of alfalfa root rot pathogenic bacteria as indicator strains.Result]5 strains of more antagonistic microorganism were obtained from No.1,No.4,No.6 and No... |
| Keywords: | Actinomyces Antagonism Alfalfa Root rot |
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