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石河子地区奶牛乳房炎病原菌分离鉴定及中药抑菌试验
引用本文:宋华容,李正国,罗瑞卿,关辉,谷新利,张飞,周霞.石河子地区奶牛乳房炎病原菌分离鉴定及中药抑菌试验[J].安徽农业科学,2009,37(9).
作者姓名:宋华容  李正国  罗瑞卿  关辉  谷新利  张飞  周霞
作者单位:1. 石河子大学动物科技学院,新疆石河子,832003
2. 石河子大学科技处,新疆石河子,832003
3. 新疆石河子市西部牧业,新疆石河子,832000
摘    要:目的]为使用中草药防治奶牛乳房炎提供理论依据。方法]采集52头奶牛的260份乳样,用乳腺炎诊断液(SMT)诊断奶牛乳房炎,并从奶样中分离鉴定出乳房炎病原菌;在此基础上,选取4株肠杆菌2、株链球菌和3株葡萄球菌进行中药及其复方的抑菌试验,根据最小抑菌浓度(MIC)和最小杀菌浓度(MBC)评价中药的体外抗菌活性。结果]从260份乳样中共分离出176株细菌,其中葡萄球菌71株,占总数的40.34%,肠杆菌62株,占总数的35.23%,链球菌25株,占总数的14.21%,这3个细菌是奶牛乳房炎的主要致病菌;75.57%的乳房炎由2种以上细菌混合感染所致。结论]奶牛乳房炎主要致病菌对单味中药产生了不同程度的耐药性,而中药复方的抑菌效果较好。

关 键 词:乳房炎  病原菌  中药  抑菌试验

Isolation and Identification on Mastitis Pathogen of Cow in Shihezi Area and Bacteriostatic Test with Chinese Herbal Medicine
SONG Hua-rong et al.Isolation and Identification on Mastitis Pathogen of Cow in Shihezi Area and Bacteriostatic Test with Chinese Herbal Medicine[J].Journal of Anhui Agricultural Sciences,2009,37(9).
Authors:SONG Hua-rong
Institution:SONG Hua-rong et al (College of Animal Science , Technology,Shihezi University,Shihezi,Xinjiang 832003)
Abstract:Objective] The aim was to provide the theoretical basis for preventing cow mastitis with Chinese herbal medicine.Method] 260 milk samples of 52 cows were collected.Using mastitis test solution(JMT) to diagnose cow mastitis and the pathogen of mastitis was isolated and identified from milk examples.On the basis of it,4 strains of enteric bacilli,2 strains of streptococcus and 3 strains of staphylococcus were selected for bacteriostatic test with Chinese herbal medicine and their compound.According to the m...
Keywords:Mastitis  Pathogen  Chinese herbal medicine  Bacteriostatic test  
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